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pACCAR16 delta crtX

pACYC184 vector-based plasmid for biosynthesis of β-carotene in E. coli (crtE, crtB, crtI, crtY).

Catalog number RDB19486
Resource name pACCAR16 delta crtX
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of β-carotene in E. coli. See Fig. 2 of Misawa, N. et al., J. Bacteriol., 177 (22): 6575-6584, 1995.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB, crtI, crtY.
Reference of insert sequence LC642801 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Pantoea ananatis crtE Pantoea ananatis crtY Pantoea ananatis crtI Pantoea ananatis crtB
    Reference sequence
      D90087.2
     

    Distribution information

    Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
    Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)
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    提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)。
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    RDB19486 pACCAR16 delta crtX Under QC test. Please contact us. DNA solution

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    How to cite this biological resource

    Materials & Methods section:

    The pACCAR16 delta crtX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19486).

    Reference section:

    Misawa, N., Satomi, Y., Kondo, K., Yokoyama, A., Kajiwara, S., Saito, T., Ohtani, T., Miki, W., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP]

    Further references such as user reports and related articles (go to bottom)

    References and tips

    Featured content

    Featured content Biosynthesis of carotenoid (English text)

    QC test results

    check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

    References

    Original, user report and related articles

    original Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP]
    reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

    2023.06.27

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