RDB19522 - CDF-MpCYP97C-MpLCYe

CDF-MpCYP97C-MpLCYe (#RDB19522)

MpCYP97C- and MpLCYe-expression plasmid for production of lutein when coexpreessd with a plasmid to biosynthsize zeinoxanthin in E. coli.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Takemura, M., Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [PubMed] [Article] [RRC of NBRP]
Test sheet	Data Sheet open

Clone info.	MpCYP97C- and MpLCYe-expression plasmid for production of lutein when coexpreessd with a plasmid to biosynthsize zeinoxanthin in E. coli. See Stable 1 of Takemura, M. et al., Synth. Biol., 6 (1): ysab012, 2021.
Vector backbone	pCDFDuet-1 (plasmid)
Selectable markers	spectinomycin
Growth conditions	LB medium including 100 mg/L of spectinomycin at 37℃
Gene/insert name	Marchantia polymorpha MpCYP97C, MpLCYe.
Reference of insert sequence	LC654938 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)。

Catalog #	Resource name	Shipping form	Fee
RDB19522	CDF-MpCYP97C-MpLCYe	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The CDF-MpCYP97C-MpLCYe was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19522).

Reference section:

Takemura, M., Kubo, A., Watanabe, A., Sakuno, H., Minobe, Y., Sahara, T., Murata, M., Araki, M., Harada, H., Terada, Y., Yaoi, K., Ohdan, K., Misawa, N., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [PubMed] [Article] [RRC of NBRP]