RDB19501 - pAC-HIEBIYZG

pAC-HIEBIYZG (#RDB19501)

pACYC184 vector-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtG).

Drawn by SnapGene® software

Sequence (full)	RDB19501hts01.seq
Publication	Misawa, N., Methods Enzymol. 670: 87-137 (2022). [link to RRC of NBRP]
Test sheet	RDB19501_B2Drp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of nostoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pACYC184 (plasmid)
Selectable markers	tetracycline
Growth remarks	For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Brevundimonas sp. strain SD212 crtG.
Reference of insert sequence	LC635756 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19501	pAC-HIEBIYZG	DNA solution

How to cite this biological resource

Materials & Methods section:

The pAC-HIEBIYZG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19501).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]