RDB19493 - pACHP-Beta

pACHP-Beta (#RDB19493)

pACYC184 vector-based plasmid for biosynthesis of β-carotene (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR16ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY).

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Takemura, M., Planta 241 (3): 699-710 (2015). PMID 25467956. [PubMed] [Article] [RRC of NBRP]
Test sheet	Data Sheet open

Clone info.	pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of β-carotene (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR16ΔcrtX with HpIDI.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pACYC184 (plasmid)
Selectable markers	chloramphenicol
Growth conditions	LB medium including 30 mg/L of Cm at 37℃
Growth remarks	For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY.
Reference of insert sequence	LC645089 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Planta. 241(3): 699-710, 2015)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Planta. 241(3): 699-710, 2015)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Planta. 241(3): 699-710, 2015)。

Catalog #	Resource name	Shipping form	Fee
RDB19493	pACHP-Beta	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pACHP-Beta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19493).

Reference section:

Takemura, M., Maoka, T., Misawa, N., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Takemura, M., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. [PubMed] [Article] [RRC of NBRP]
reference	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP]