Resource data sheet
Prev.
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pAC-HIM

pACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM).

Catalog number RDB19504
Resource name pAC-HIM
Clone info. pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See SFig. 1 of Takemura, M. et al., Microb. Cell Fact., 20 (1): 194-205, 2021.
Comment Expression was confirmed by the depositor: HPLC analysis of the E. coli extract.
Vector backbone pACYC184 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Leuconostoc mesenteroides subsp. mesenteroides crtM
Reference of insert sequence LC647532 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Leuconostoc mesenteroides subsp. mesenteroides crtM
        Reference sequence
          AB019034
         
        Sequence (full) RDB19504hts01.seqcheck assembled from experimentally sequenced data
        Test sheet RDB19504_B2Drp1-1.pdf 

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)
        Ordering
        Order form [Credit Card Payment]  [Bank Transfer Payment]
        MTA, for use for not-for-profit academic purpose [Word]
        Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
        提供案内 (日本国内) [open/close]

        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)。
        提供依頼
        提供依頼書 [Word]
        提供同意書 (MTA, 非営利学術目的用)[Word]
        手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB19504 pAC-HIM DNA solution

        How to cite this biological resource

        Materials & Methods section:

        The pAC-HIM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19504).

        Reference section:

        Takemura, M., Takagi, C., Aikawa, M., Araki, K., Choi, S.K., Itaya, M., Shindo, K., Misawa, N., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)

        Tips

        Featured content

        Featured content Biosynthesis of carotenoid (English text)

        References

        Original, user report and related articles

        original Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]
        reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

        2024.03.08

        HTML_filter_RDB_240127.pl