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pAC-HIM

pACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM).

Catalog number RDB19504
Resource name pAC-HIM
Clone info. pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See SFig. 1 of Takemura, M. et al., Microb. Cell Fact., 20 (1): 194-205, 2021.
Comment Expression was confirmed by the depositor: HPLC analysis of the E. coli extract.
Vector backbone pACYC184 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Leuconostoc mesenteroides subsp. mesenteroides crtM
Reference of insert sequence LC647532 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19504hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19504_B2Drp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)。
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RDB19504 pAC-HIM DNA solution


How to cite this biological resource

Materials & Methods section:

The pAC-HIM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19504).

Reference section:

Takemura, M., Takagi, C., Aikawa, M., Araki, K., Choi, S.K., Itaya, M., Shindo, K., Misawa, N., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]