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pAC-HIM

pACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM).

Catalog number RDB19504
Resource name pAC-HIM
Clone info. pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See SFig. 1 of Takemura, M. et al., Microb. Cell Fact., 20 (1): 194-205, 2021.
Comment Expression was confirmed by the depositor: HPLC analysis of the E. coli extract.
Vector backbone pACYC184 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Leuconostoc mesenteroides subsp. mesenteroides crtM
Reference of insert sequence LC647532 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Leuconostoc mesenteroides subsp. mesenteroides crtM
        Reference sequence
          AB019034
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)。
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        RDB19504 pAC-HIM Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        How to cite this biological resource

        Materials & Methods section:

        The pAC-HIM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19504).

        Reference section:

        Takemura, M., Takagi, C., Aikawa, M., Araki, K., Choi, S.K., Itaya, M., Shindo, K., Misawa, N., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)

        References and tips

        Featured content

        Featured content Biosynthesis of carotenoid (English text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]
        reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

        2023.06.27

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