pACYC184 vector-based plasmid for biosynthesis of 15-cis-4,4'-diapophytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM).
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External Database Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Leuconostoc mesenteroides subsp. mesenteroides crtM Reference sequence AB019034 |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021) |
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Ordering | Order form [Credit Card Payment ![]() ![]() Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)。 |
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提供依頼 | 提供依頼書 [Word ![]() 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB19504 | pAC-HIM | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The pAC-HIM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19504). |
Reference section:
Takemura, M., Takagi, C., Aikawa, M., Araki, K., Choi, S.K., Itaya, M., Shindo, K., Misawa, N., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Biosynthesis of carotenoid (English text) |
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Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP] |
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reference | Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
2023.06.27
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