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pACHP-Lyc (#RDB19492)

pACYC184 vector-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB with HpIDI (HpIDI, crtE, crtB, crtI).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)
GenBank Flat File Format open
SnapGene file download
Publication Takemura, M., BMC Zoology 6:19 (2021). [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB (RDB19484) with HpIDI. See SFig. 3 of Takemura, M. et al., BMC Zoology, 6: 19, 2021.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth conditions LB medium including 30 mg/L of Cm at 37℃
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI.
Reference of insert sequence LC645212 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. BMC Zoology. 6:19, 2021).
The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
RECIPIENT must contact the Center for Academic and Industrial Collaboration, Ishikawa Prefectural University (Kanae Sawamura, Coordinator: k-sawamu@ishikawa-pu.ac.jp) in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control.
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必要書類
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. BMC Zoology. 6:19, 2021)。
基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。
利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、石川県立大学・産学官連携学術交流センター(Tel 076-227-7566; Fax 076-227-7557 (コーディネーター 澤村奏絵, k-sawamu@ishikawa-pu.ac.jp))と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。

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RDB19492 pACHP-Lyc DNA solution


How to cite this biological resource

Materials & Methods section:

The pACHP-Lyc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19492).

Reference section:

Takemura, M., Maoka, T., Koyanagi, T., Kawase, N., Nishida, R., Tsuchida, T., Hironaka, M., Ueda, T., Misawa, N., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). [Article] [RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP]