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pACHP-Lyc

pACYC184 vector-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB with HpIDI (HpIDI, crtE, crtB, crtI).

Catalog number RDB19492
Resource name pACHP-Lyc
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of lycopene (addtionally including the HpIDI gene) in E. coli. Plasmid pACCRT-EIB with HpIDI. See SFig. 3 of Takemura, M. et al., BMC Zoology, 6: 19, 2021.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI.
Reference of insert sequence LC645212 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Pantoea ananatis crtE Pantoea ananatis crtI Pantoea ananatis crtB
    Reference sequence
      AB019034, D90087.2
     

    Distribution information

    Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
    Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. BMC Zoology. 6:19, 2021)
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    提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. BMC Zoology. 6:19, 2021)。
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    RDB19492 pACHP-Lyc Under QC test. Please contact us. DNA solution

    check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

    How to cite this biological resource

    Materials & Methods section:

    The pACHP-Lyc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19492).

    Reference section:

    Takemura, M., Maoka, T., Koyanagi, T., Kawase, N., Nishida, R., Tsuchida, T., Hironaka, M., Ueda, T., Misawa, N., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). [link to RRC of NBRP]

    Further references such as user reports and related articles (go to bottom)

    References and tips

    Featured content

    Featured content Biosynthesis of carotenoid (English text)

    QC test results

    check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

    References

    Original, user report and related articles

    original Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). [link to RRC of NBRP]
    reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

    2023.06.27

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