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pETDuet-MpLCYb/MpLCYe (#RDB19519)

MpLCYb- and MpLCYe-expression plasmid for production of α-carotene (and β-carotene) when coexpressed with a plasmid to biosynthesize lycopene in E. coli.


Drawn by SnapGene® software
Sequence (full) RDB19519hts01.seq
Publication Takemura, M., Planta 241 (3): 699-710 (2015). [link to RRC of NBRP]
Test sheet RDB19519_B2Drp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. MpLCYb- and MpLCYe-expression plasmid for production of α-carotene (and β-carotene) when coexpressed with a plasmid to biosynthesize lycopene in E. coli.
Vector backbone pETDuet-1 (plasmid)
Selectable markers ampicillin
Gene/insert name Marchantia polymorpha MpLCYb, MpLCYe.
Reference of insert sequence LC654696 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Planta, 241(3): 699-710, 2015)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Planta, 241(3): 699-710, 2015)。

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RDB19519 pETDuet-MpLCYb/MpLCYe DNA solution

How to cite this biological resource

Materials & Methods section:

The pETDuet-MpLCYb/MpLCYe was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19519).

Reference section:

Takemura, M., Maoka, T., Misawa, N., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takemura, M., Biosynthetic routes of hydroxylated carotenoids (xanthophylls) in Marchantia polymorpha, and production of novel and rare xanthophylls through pathway engineering in Escherichia coli. Planta 241 (3): 699-710 (2015). PMID 25467956. [link to RRC of NBRP]