RDB19512 - pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg

pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg (#RDB19512)

pRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE).

Drawn by SnapGene® software

Sequence (full)	RDB19512hts01.seq
Publication	Takemura, M., Synth. Biol. (Oxf) 6 (1): ysab012 (2021). [link to RRC of NBRP]
Test sheet	RDB19512_B2Drp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	pRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli. See Stable 1 of Takemura, M. et al., Synth. Biol., 6 (1): ysab012, 2021.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pRK404 (plasmid)
Selectable markers	tetracycline
Growth remarks	For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI; Marchantia polymorpha MpLCYb, MpLCYe; Pantoea ananatis crtZ; Pantoea agglomerans Pag-crtE.
Reference of insert sequence	LC654937 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19512	pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg	DNA solution

How to cite this biological resource

Materials & Methods section:

The pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19512).

Reference section:

Takemura, M., Kubo, A., Watanabe, A., Sakuno, H., Minobe, Y., Sahara, T., Murata, M., Araki, M., Harada, H., Terada, Y., Yaoi, K., Ohdan, K., Misawa, N., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]
reference	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]