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pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg

pRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, MpLCYb, MpLCYe, crtZ, Pag-crtE).

Catalog number RDB19512
Resource name pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg
Clone info. pRK404 vector-based plasmid for biosynthesis of zeinoxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli. See Stable 1 of Takemura, M. et al., Synth. Biol., 6 (1): ysab012, 2021.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pRK404 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI; Marchantia polymorpha MpLCYb, MpLCYe; Pantoea ananatis crtZ; Pantoea agglomerans Pag-crtE.
Reference of insert sequence LC654937 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19512hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19512_B2Drp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)。
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RDB19512 pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg DNA solution


How to cite this biological resource

Materials & Methods section:

The pRK-HIEBI-MpLCYb delta TP-MpLCYe-Z-Epg was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19512).

Reference section:

Takemura, M., Kubo, A., Watanabe, A., Sakuno, H., Minobe, Y., Sahara, T., Murata, M., Araki, M., Harada, H., Terada, Y., Yaoi, K., Ohdan, K., Misawa, N., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]