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pAC-HIMN (#RDB19505)

pACYC184 vector-based plasmid for biosynthesis of 4,4'-diaponeurosporene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtM, crtN).


Drawn by SnapGene® software
Sequence (full) RDB19505hts01.seq
Publication Takemura, M., Microb. Cell Fact. 20 (1): 194 (2021). [link to RRC of NBRP]
Test sheet RDB19505_B2Drp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of 4,4'-diaponeurosporene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See SFig. 1 of Takemura, M. et al., Microb. Cell Fact., 20 (1): 194-205, 2021.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Leuconostoc mesenteroides subsp. mesenteroides crtM, crtN
Reference of insert sequence LC647533 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Microb. Cell Fact. 20(1): 194, 2021)。

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RDB19505 pAC-HIMN DNA solution

How to cite this biological resource

Materials & Methods section:

The pAC-HIMN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19505).

Reference section:

Takemura, M., Takagi, C., Aikawa, M., Araki, K., Choi, S.K., Itaya, M., Shindo, K., Misawa, N., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takemura, M., Heterologous production of novel and rare C30-carotenoids using Planococcus carotenoid biosynthesis genes. Microb. Cell Fact. 20 (1): 194 (2021). PMID 34627253. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]