pRK404 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ).
Drawn by SnapGene® software |
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Clone info. | pRK404 vector-based plasmid for biosynthesis of zeaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli. |
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Comment | Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation. |
Vector backbone | pRK404 (plasmid) |
Selectable markers | tetracycline |
Growth remarks | For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃ |
Gene/insert name | Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ. |
Reference of insert sequence | LC637572 (DDBJ accession number) |
Depositor|Developer | Misawa, Norihiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022) |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB19509 | pRK-HIEBIYZ | DNA solution |
Materials & Methods section:
The pRK-HIEBIYZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19509). |
Reference section:
Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP] |
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