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pACCRT-EB

pACYC184 vector-based plasmid for biosynthesis of phytoene in E. coli.

Catalog number RDB19483
Resource name pACCRT-EB
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of phytoene in E. coli. See Fig. 2 of Misawa, N. et al., J. Bacteriol., 177 (22): 6575-6584, 1995.
Comment Expression was confirmed by the depositor: HPLC analysis of the E. coli extract.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB.
Reference of insert sequence LC642803 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Pantoea ananatis crtE Pantoea ananatis crtB
        Reference sequence
          D90087.2
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)。
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        RDB19483 pACCRT-EB Under QC test. Please contact us. DNA solution

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        How to cite this biological resource

        Materials & Methods section:

        The pACCRT-EB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19483).

        Reference section:

        Misawa, N., Satomi, Y., Kondo, K., Yokoyama, A., Kajiwara, S., Saito, T., Ohtani, T., Miki, W., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436.

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        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References

        Original, user report and related articles

        original Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436.
        reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847.

        2023.05.22

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