Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pRK-HIEBIYZW

pRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW).

Catalog number RDB19510
Resource name pRK-HIEBIYZW
Clone info. pRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone pRK404 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Paracoccus sp. strain N81106 crtW.
Reference of insert sequence LC637573 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank

External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Pantoea ananatis crtI Pantoea ananatis crtY Pantoea ananatis crtZ Paracoccus sp. (strain N81106/MBIC 01143) crtW
Reference sequence
  AB019034, D90087.2, D58420.2
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)
Ordering
Order form [Credit Card Payment]  [Bank Transfer Payment]
Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
提供案内 (日本国内) [open/close]

提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。
提供依頼
提供依頼書 [Word]
提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19510 pRK-HIEBIYZW Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pRK-HIEBIYZW was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19510).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References and tips

Featured content

Featured content Biosynthesis of carotenoid (English text)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


References

Original, user report and related articles

original Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

2023.06.27

GNP_filter3_RDBDEP_html_230615.pl