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pRK-HIEBIYZW (#RDB19510)

pRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, crtW).


Drawn by SnapGene® software
Sequence (full) RDB19510hts01.seq
Publication Misawa, N., Methods Enzymol. 670: 87-137 (2022). [link to RRC of NBRP]
Test sheet RDB19510_B2Drp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. pRK404 vector-based plasmid for biosynthesis of astaxanthin (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone pRK404 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Paracoccus sp. strain N81106 crtW.
Reference of insert sequence LC637573 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。

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RDB19510 pRK-HIEBIYZW DNA solution

How to cite this biological resource

Materials & Methods section:

The pRK-HIEBIYZW was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19510).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]