RDB19494 - pACHP-Zea

pACHP-Zea (#RDB19494)

pACYC184 vector-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY, crtZ).

Drawn by SnapGene® software

Sequence (full)	RDB19494hts01.seq
Publication	Takemura, M., Tetrahedron Letters 56(44): 6063-6065 (2015). [link to RRC of NBRP]
Test sheet	RDB19494_B2Drp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25ΔcrtX with HpIDI.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pACYC184 (plasmid)
Selectable markers	chloramphenicol
Growth remarks	For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ.
Reference of insert sequence	LC645090 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al, Tetrahedron Lett., 56(44), 6063-6065, 2015)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al, Tetrahedron Lett., 56(44), 6063-6065, 2015)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al, Tetrahedron Lett., 56(44), 6063-6065, 2015)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19494	pACHP-Zea	DNA solution

How to cite this biological resource

Materials & Methods section:

The pACHP-Zea was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19494).

Reference section:

Takemura, M., Maoka, T., Osawa, A., Higashinaka, H., Shimada, H., Shindo, K., Misawa, N., (6E) and (6Z)-9′-Aporhodoxanthinone, novel carotenoids produced in zeaxanthin-synthesizing-Escherichia coli by redox stress. Tetrahedron Letters 56(44): 6063-6065 (2015). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Takemura, M., (6E) and (6Z)-9′-Aporhodoxanthinone, novel carotenoids produced in zeaxanthin-synthesizing-Escherichia coli by redox stress. Tetrahedron Letters 56(44): 6063-6065 (2015). [link to RRC of NBRP]
reference	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]