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pACHP-Zea

pACYC184 vector-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25ΔcrtX with HpIDI (HpIDI, crtE, crtB, crtI, crtY, crtZ).

Catalog number RDB19494
Resource name pACHP-Zea
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of zeaxanthin (addtionally including the HpIDI gene) in E. coli. plasmid pACCAR25ΔcrtX with HpIDI.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ.
Reference of insert sequence LC645090 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19494hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19494_B2Drp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al, Tetrahedron Lett., 56(44), 6063-6065, 2015)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al, Tetrahedron Lett., 56(44), 6063-6065, 2015)。
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RDB19494 pACHP-Zea DNA solution


How to cite this biological resource

Materials & Methods section:

The pACHP-Zea was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19494).

Reference section:

Takemura, M., Maoka, T., Osawa, A., Higashinaka, H., Shimada, H., Shindo, K., Misawa, N., (6E) and (6Z)-9′-Aporhodoxanthinone, novel carotenoids produced in zeaxanthin-synthesizing-Escherichia coli by redox stress. Tetrahedron Letters 56(44): 6063-6065 (2015). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Takemura, M., (6E) and (6Z)-9′-Aporhodoxanthinone, novel carotenoids produced in zeaxanthin-synthesizing-Escherichia coli by redox stress. Tetrahedron Letters 56(44): 6063-6065 (2015). [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]