Resource data sheet
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pRK-HIEB

pRK404 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB).

Catalog number RDB19506
Resource name pRK-HIEB
Clone info. pRK404 vector-based plasmid for biosynthesis of phytoene (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment Expression was confirmed by the depositor: HPLC analysis of the E. coli extract.
Vector backbone pRK404 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB
Reference of insert sequence LC637569 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19506hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19506_B3Iqp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。
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RDB19506 pRK-HIEB DNA solution


How to cite this biological resource

Materials & Methods section:

The pRK-HIEB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19506).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]