RDB19514 - pRK-HIEBIYZ-FhCCD2-NcALD8

pRK-HIEBIYZ-FhCCD2-NcALD8 (#RDB19514)

pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8).

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Misawa, N., Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP]
Test sheet	Data Sheet open

Clone info.	pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pRK404 (plasmid)
Selectable markers	tetracycline
Growth conditions	LB medium including 12.5 mg/L of Tc at 37℃
Growth remarks	For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Freesia hybrida FhCCD2; Neurospora crassa NcALD8.
Reference of insert sequence	LC654940 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022).
The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
RECIPIENT must contact the Center for Academic and Industrial Collaboration, Ishikawa Prefectural University (Kanae Sawamura, Coordinator: k-sawamu@ishikawa-pu.ac.jp) in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022). The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes. RECIPIENT must contact the Center for Academic and Industrial Collaboration, Ishikawa Prefectural University (Kanae Sawamura, Coordinator: k-sawamu@ishikawa-pu.ac.jp) in the case of application for any patents or commercial use() based on the results from the use of the BIOLOGICAL RESOURCE. ()Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動()を行う場合は、石川県立大学・産学官連携学術交流センター（Tel 076-227-7566; Fax 076-227-7557 (コーディネーター澤村奏絵, k-sawamu@ishikawa-pu.ac.jp)）と別途協議を行う。()商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。
基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。
利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、石川県立大学・産学官連携学術交流センター（Tel 076-227-7566; Fax 076-227-7557 (コーディネーター澤村奏絵, k-sawamu@ishikawa-pu.ac.jp)）と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。

Catalog #	Resource name	Shipping form	Fee
RDB19514	pRK-HIEBIYZ-FhCCD2-NcALD8	DNA solution

How to cite this biological resource

Materials & Methods section:

The pRK-HIEBIYZ-FhCCD2-NcALD8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19514).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP]