RDB19514 - pRK-HIEBIYZ-FhCCD2-NcALD8

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pRK-HIEBIYZ-FhCCD2-NcALD8

pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8).

Catalog number	RDB19514
Resource name	pRK-HIEBIYZ-FhCCD2-NcALD8
Clone info.	pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pRK404 (plasmid)
Selectable markers	tetracycline
Growth remarks	For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Freesia hybrida FhCCD2; Neurospora crassa NcALD8.
Reference of insert sequence	LC654940 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Other clones in our bank

External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI

KEGG Orthology

Pantoea ananatis crtY

KEGG Orthology

Pantoea ananatis crtZ

KEGG Orthology

Freesia hybrida CCD2

KEGG Orthology

Neurospora crassa NcALD8.

KEGG Orthology

Reference sequence
AB019034, D90087.2

Sequence (full)	RDB19514hts01.seq assembled from experimentally sequenced data
Test sheet	RDB19514_B2Drp1-1.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)

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提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19514	pRK-HIEBIYZ-FhCCD2-NcALD8	DNA solution

How to cite this biological resource

Materials & Methods section:

The pRK-HIEBIYZ-FhCCD2-NcALD8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19514).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

Featured content	Biosynthesis of carotenoid (English text)

References

Original, user report and related articles

original	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

2023.07.13

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