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pRK-HIEBIYZ-FhCCD2-NcALD8

pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, crtY, crtZ, FhCCD2, NcALD8).

Catalog number RDB19514
Resource name pRK-HIEBIYZ-FhCCD2-NcALD8
Clone info. pRK404 vector-based plasmid for biosynthesis of crocetin (additionally including HpIDI) under the direction of the tac promoter in E. coli.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pRK404 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Freesia hybrida FhCCD2; Neurospora crassa NcALD8.
Reference of insert sequence LC654940 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19514hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19514_B2Drp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. Method in Enzymology, Vol 670, 2022)。
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RDB19514 pRK-HIEBIYZ-FhCCD2-NcALD8 DNA solution


How to cite this biological resource

Materials & Methods section:

The pRK-HIEBIYZ-FhCCD2-NcALD8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19514).

Reference section:

Misawa, N., Maoka, T., Takemura, M., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]