RDB19487 - pACCAR25 delta crtX

pACCAR25 delta crtX (#RDB19487)

pACYC184 vector-based plasmid for biosynthesis of zeaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ).

Drawn by SnapGene® software

Sequence (full)	RDB19487hts01.seq
Publication	Misawa, N., J. Bacteriol. 177 (22): 6575-6584 (1995). [link to RRC of NBRP]
Test sheet	RDB19487_B2Drp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of zeaxanthin in E. coli. See Fig. 2 of Misawa, N., et al. J. Bacteriol., 177 (22): 6575-6584, 1995.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pACYC184 (plasmid)
Selectable markers	chloramphenicol
Growth remarks	For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name	Pantoea ananatis crtE, crtB, crtI, crtY, crtZ.
Reference of insert sequence	LC642800 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19487	pACCAR25 delta crtX	DNA solution

How to cite this biological resource

Materials & Methods section:

The pACCAR25 delta crtX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19487).

Reference section:

Misawa, N., Satomi, Y., Kondo, K., Yokoyama, A., Kajiwara, S., Saito, T., Ohtani, T., Miki, W., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP]
reference	Matsumoto, W., Carotenoid productivity in human intestinal bacteria Eubacterium limosum and Leuconostoc mesenteroides with functional analysis of their carotenoid biosynthesis genes. Engineering Microbiology 4 (2): 100147 (2024).
reference	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]