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pACCAR25 delta crtX

pACYC184 vector-based plasmid for biosynthesis of zeaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ).

Catalog number RDB19487
Resource name pACCAR25 delta crtX
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of zeaxanthin in E. coli. See Fig. 2 of Misawa, N., et al. J. Bacteriol., 177 (22): 6575-6584, 1995.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB, crtI, crtY, crtZ.
Reference of insert sequence LC642800 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19487hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19487_B2Drp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al. J. Bacteriol. 177(22): 6575-6584, 1995)。
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RDB19487 pACCAR25 delta crtX DNA solution


How to cite this biological resource

Materials & Methods section:

The pACCAR25 delta crtX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19487).

Reference section:

Misawa, N., Satomi, Y., Kondo, K., Yokoyama, A., Kajiwara, S., Saito, T., Ohtani, T., Miki, W., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Misawa, N., Structure and functional analysis of a marine bacterial carotenoid biosynthesis gene cluster and astaxanthin biosynthetic pathway proposed at the gene level. J. Bacteriol. 177 (22): 6575-6584 (1995). PMID 7592436. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]