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pUC-CaZEP (#RDB19518)

CaZEP-expression plasmid for production of violaxanthin and antheraxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Takemura, M., Appl. Microbiol. Biotechnol. 103 (23-24): 9393-9399 (2019). PMID 31673744. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. CaZEP-expression plasmid for production of violaxanthin and antheraxanthin when coexpressed with a plasmid to biosynthesize zeaxanthin in E. coli.
Vector backbone pUC18 (plasmid)
Selectable markers ampicillin
Growth conditions LB medium including 75 mg/L of ampicillin at 37℃
Gene/insert name Capsicum annuum CaZEP.
Reference of insert sequence LC656480 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Appl. Microbiol. Biotechnol. 103(23-24): 9393-9399, 2019)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Appl. Microbiol. Biotechnol. 103(23-24): 9393-9399, 2019)。

Catalog # Resource name Shipping form Fee
RDB19518 pUC-CaZEP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pUC-CaZEP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19518).

Reference section:

Takemura, M., Kubo, A., Higuchi, Y., Maoka, T., Sahara, T., Yaoi, K., Ohdan, K., Umeno, D., Misawa, N., Pathway engineering for efficient biosynthesis of violaxanthin in Escherichia coli. Appl. Microbiol. Biotechnol. 103 (23-24): 9393-9399 (2019). PMID 31673744. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takemura, M., Pathway engineering for efficient biosynthesis of violaxanthin in Escherichia coli. Appl. Microbiol. Biotechnol. 103 (23-24): 9393-9399 (2019). PMID 31673744. [PubMed] [Article] [RRC of NBRP]