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pAC-Cantha

pACYC184 vector-based plasmid for biosynthesis of canthaxanthin in E. coli (crtE, crtB, crtI, crtY, crtW).

Catalog number RDB19488
Resource name pAC-Cantha
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of canthaxanthin in E. coli. See Table 1 of Nishida, Y. et al., Appl. Environ. Microbiol., 71 (8): 4286-4289, 2005.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB, crtI, crtY; Paracoccus sp. strain N81106 crtW.
Reference of insert sequence LC643778 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Paracoccus sp. (strain N81106/MBIC 01143) crtW Pantoea ananatis crtE Pantoea ananatis crtY Pantoea ananatis crtI Pantoea ananatis crtB
Reference sequence
  D90087.2, D58420.2
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)。
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RDB19488 pAC-Cantha Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pAC-Cantha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19488).

Reference section:

Nishida, Y., Adachi, K., Kasai, H., Shizuri, Y., Shindo, K., Sawabe, A., Komemushi, S., Miki, W., Misawa, N., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

Featured content Biosynthesis of carotenoid (English text)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

2023.06.27

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