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pAC-Cantha

pACYC184 vector-based plasmid for biosynthesis of canthaxanthin in E. coli (crtE, crtB, crtI, crtY, crtW).

Catalog number RDB19488
Resource name pAC-Cantha
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of canthaxanthin in E. coli. See Table 1 of Nishida, Y. et al., Appl. Environ. Microbiol., 71 (8): 4286-4289, 2005.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB, crtI, crtY; Paracoccus sp. strain N81106 crtW.
Reference of insert sequence LC643778 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB19488hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19488_B2Drp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)。
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RDB19488 pAC-Cantha DNA solution


How to cite this biological resource

Materials & Methods section:

The pAC-Cantha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19488).

Reference section:

Nishida, Y., Adachi, K., Kasai, H., Shizuri, Y., Shindo, K., Sawabe, A., Komemushi, S., Miki, W., Misawa, N., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]