pACYC184 vector-based plasmid for biosynthesis of canthaxanthin in E. coli (crtE, crtB, crtI, crtY, crtW).
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Clone info. | pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of canthaxanthin in E. coli. See Table 1 of Nishida, Y. et al., Appl. Environ. Microbiol., 71 (8): 4286-4289, 2005. |
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Comment | Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation. |
Vector backbone | pACYC184 (plasmid) |
Selectable markers | chloramphenicol |
Growth conditions | LB medium including 30 mg/L of Cm at 37℃ |
Growth remarks | For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃ |
Gene/insert name | Pantoea ananatis crtE, crtB, crtI, crtY; Paracoccus sp. strain N81106 crtW. |
Reference of insert sequence | LC643778 (DDBJ accession number) |
Depositor|Developer | Misawa, Norihiko | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005). The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes. RECIPIENT must contact the Intellectual Property Strategy Department, Kirin Holdings Company, Limited in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)。 基礎検討の使用に関しては,営利,非営利機関等の利用者制限は定めない。 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、キリンホールディングス株式会社(知財戦略推進部; 03-6837-7000 代表)と別途協議を行う。(*)商用活動とは,本件リソースが製品の一部に組み込まれる場合,本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。 |
Catalog # | Resource name | Shipping form | Fee |
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RDB19488 | pAC-Cantha | DNA solution |
Materials & Methods section:
The pAC-Cantha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19488). |
Reference section:
Nishida, Y., Adachi, K., Kasai, H., Shizuri, Y., Shindo, K., Sawabe, A., Komemushi, S., Miki, W., Misawa, N., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [PubMed] [Article] [RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [PubMed] [Article] [RRC of NBRP] |
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reference | Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [PubMed] [Article] [RRC of NBRP] |
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