RDB19502 - pAC-HIEBI-ApCrtYB3

pAC-HIEBI-ApCrtYB3 (#RDB19502)

pACYC184 vector-based plasmid for biosynthesis of β,γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, ApCrtYB3).

Drawn by SnapGene® software

Sequence (full)	RDB19502hts01.seq
Publication	Takemura, M., BMC Zoology 6:19 (2021). [link to RRC of NBRP]
Test sheet	RDB19502_B2Drp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of β,γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See SFig. 3 of Takemura, M. et al., BMC Zoology, 6: 19, 2021.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone	pACYC184 (plasmid)
Selectable markers	tetracycline
Growth remarks	For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name	Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI; Acyrthosiphon pisum ApCrtYB3.
Reference of insert sequence	LC647213 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. BMC Zoology. 6:19, 2021)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. BMC Zoology. 6:19, 2021)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. BMC Zoology. 6:19, 2021)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19502	pAC-HIEBI-ApCrtYB3	DNA solution

How to cite this biological resource

Materials & Methods section:

The pAC-HIEBI-ApCrtYB3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19502).

Reference section:

Takemura, M., Maoka, T., Koyanagi, T., Kawase, N., Nishida, R., Tsuchida, T., Hironaka, M., Ueda, T., Misawa, N., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Takemura, M., Elucidation of the whole carotenoid biosynthetic pathway of aphids at the gene level and arthropodal food chain involving aphids and the red dragonfly. BMC Zoology 6:19 (2021). [link to RRC of NBRP]
reference	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]