RDB19489 - pAC-Asta

pAC-Asta (#RDB19489)

pACYC184 vector-based plasmid for biosynthesis of astaxanthin in E. coli (crtE, crtB, crtI, crtY, crtZ, crtW).

Drawn by SnapGene® software

Sequence (full)	RDB19489hts01.seq
Publication	Nishida, Y., Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). [link to RRC of NBRP]
Test sheet	RDB19489_B2Drp1-1.pdf

Assembled from experimentally sequenced data.

Clone info.	pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of astaxanthin in E. coli. See Table 1 of Nishida, Y. et al., Appl. Environ. Microbiol., 71 (8): 4286-4289, 2005.
Comment	Expression was confirmed by the depositor: formation of E. coli colonies with red pigmentation.
Vector backbone	pACYC184 (plasmid)
Selectable markers	chloramphenicol
Growth remarks	For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name	Pantoea ananatis crtE, crtB, crtI, crtY, crtZ; Paracoccus sp. strain N81106 crtW.
Reference of insert sequence	LC643777 (DDBJ accession number)
Depositor\|Developer	Misawa, Norihiko \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishida, Y. et al. Appl. Environ. Microbiol. 71(8): 4286-4296, 2005)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19489	pAC-Asta	DNA solution

How to cite this biological resource

Materials & Methods section:

The pAC-Asta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19489).

Reference section:

Nishida, Y., Adachi, K., Kasai, H., Shizuri, Y., Shindo, K., Sawabe, A., Komemushi, S., Miki, W., Misawa, N., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Nishida, Y., Elucidation of a carotenoid biosynthesis gene cluster encoding a novel enzyme, 2,2'-beta-hydroxylase, from Brevundimonas sp. strain SD212 and combinatorial biosynthesis of new or rare xanthophylls. Appl. Environ. Microbiol. 71 (8): 4286-4296 (2005). PMID 16085816. [link to RRC of NBRP]
reference	Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]