Resource data sheet
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pCAR25 (#RDB20278)

Expression vector for production of zeaxanthin diglucoside.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Misawa, N., J. Bacteriol. 172 (12): 6704-6712 (1990). PMID 2254247. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector for production of pUC19 vector (Ap resistance)-based plasmid for biosynthesis of zeaxanthin diglucoside. See Fig. 1 of Misawa, N. et al., J. Bacteriol., 172: 6704, 1990 (DOI 10.1128/jb.172.12.6704-6712.1990).
Comment Complete nucleotide sequence of pCAR25: LC789199.
Vector backbone pUC19 (plasmid)
Growth conditions LB medium including 75 mg/L of ampicillin at 37℃
Growth remarks Transformants grow slowly in amplification of the plasmid.
For carotenoid production, e.g., 2YT medium including 75 mg/L of ampicillin at 20℃.
Gene/insert name Pantoea ananatis crtE, crtX, crtY, crtI, crtB, crtZ.
Reference of insert sequence LC789199 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Misawa, N. et al., J. Bacteriol., 172(12), 6704-6712.1990).
The RECIPIENT of any organization including for-profit or nonprofit organizations may intend to use the BIOLOGICAL RESOURCE for basic researches with such purposes.
RECIPIENT must contact the Intellectual Property Strategy Department, Kirin Holdings Company, Limited in the case of application for any patents or commercial use(*) based on the results from the use of the BIOLOGICAL RESOURCE. (*)Commercial use shall include: (1) incorporation of the BIOLOGICAL RESOURCE into products for sale; (2) the use of to the BIOLOGICAL RESOURCE for screening candidate compounds; (3) the use of to the BIOLOGICAL RESOURCE for quality control.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al., J. Bacteriol., 172(12), 6704-6712.1990)。
基礎検討の使用に関しては，営利，非営利機関等の利用者制限は定めない。
利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び商業活動(*)を行う場合は、キリンホールディングス株式会社(知財戦略推進部; 03-6837-7000 代表)と別途協議を行う。(*)商用活動とは，本件リソースが製品の一部に組み込まれる場合，本件リソースを利用して製品となる候補物を探索または製品の品質管理を行う場合等とする。

Catalog # Resource name Shipping form Fee
RDB20278 pCAR25 DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAR25 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20278).

Reference section:

Misawa, N., Nakagawa, M., Kobayashi, K., Yamano, S., Izawa, Y., Nakamura, K., Harashima, K., Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12): 6704-6712 (1990). PMID 2254247. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Misawa, N., Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12): 6704-6712 (1990). PMID 2254247. [PubMed] [Article] [RRC of NBRP]