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pCAR25

Expression vector for production of zeaxanthin diglucoside.

Catalog number RDB20278
Resource name pCAR25
Clone info. Expression vector for production of pUC19 vector (Ap resistance)-based plasmid for biosynthesis of zeaxanthin diglucoside. See Fig. 1 of Misawa, N. et al., J. Bacteriol., 172: 6704, 1990 (DOI 10.1128/jb.172.12.6704-6712.1990).
Comment Complete nucleotide sequence of pCAR25: LC789199.
Vector backbone pUC19 (plasmid)
Growth remarks Transformants grow slowly in amplification of the plasmid.
For carotenoid production, e.g., 2YT medium including 75 mg/L of ampicillin at 20℃.
Gene/insert name Pantoea ananatis crtE, crtX, crtY, crtI, crtB, crtZ.
Reference of insert sequence LC789199 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
 
Sequence (full) RDB20278hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB20278C0p1-1.pdf 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Misawa, N. et al., J. Bacteriol., 172(12), 6704-6712.1990)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Misawa, N. et al., J. Bacteriol., 172(12), 6704-6712.1990)。

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RDB20278 pCAR25 DNA solution

How to cite this biological resource

Materials & Methods section:

The pCAR25 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20278).

Reference section:

Misawa, N., Nakagawa, M., Kobayashi, K., Yamano, S., Izawa, Y., Nakamura, K., Harashima, K., Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12): 6704-6712 (1990). PMID 2254247. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Misawa, N., Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12): 6704-6712 (1990). PMID 2254247. [link to RRC of NBRP]