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pACCRT-EB-RcI (#RDB19485)

pACYC184 vector-based plasmid for biosynthesis of neurosporene in E. coli (crtE, crtB, Rc-crtI).


Drawn by SnapGene® software
Sequence (full) RDB19485hts01.seq
Publication Takaichi, S., Eur. J. Biochem. 241 (1): 291-296 (1996). [link to RRC of NBRP]
Test sheet RDB19485_B2Drp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of neurosporene in E. coli. See Fig. 1 of Takaichi, S. et al., Eur. J. Biochem., 241 (1): 291-296, 1996.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB; Rhodobacter capsulatus Rc-crtI.
Reference of insert sequence LC643779 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takaichi, S. et al. Eur. J. Biochem. 241(1): 291-296, 1996)
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takaichi, S. et al. Eur. J. Biochem. 241(1): 291-296, 1996)。

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RDB19485 pACCRT-EB-RcI DNA solution

How to cite this biological resource

Materials & Methods section:

The pACCRT-EB-RcI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19485).

Reference section:

Takaichi, S., Sandmann, G., Schnurr, G., Satomi, Y., Suzuki, A., Misawa, N., The carotenoid 7,8-dihydro-psi end group can be cyclized by the lycopene cyclases from the bacterium Erwinia uredovora and the higher plant Capsicum annuum. Eur. J. Biochem. 241 (1): 291-296 (1996). PMID 8898919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takaichi, S., The carotenoid 7,8-dihydro-psi end group can be cyclized by the lycopene cyclases from the bacterium Erwinia uredovora and the higher plant Capsicum annuum. Eur. J. Biochem. 241 (1): 291-296 (1996). PMID 8898919. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]