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pACCRT-EB-RcI

pACYC184 vector-based plasmid for biosynthesis of neurosporene in E. coli (crtE, crtB, Rc-crtI).

Catalog number RDB19485
Resource name pACCRT-EB-RcI
Clone info. pACYC184 vector (Cm resistance)-based plasmid for biosynthesis of neurosporene in E. coli. See Fig. 1 of Takaichi, S. et al., Eur. J. Biochem., 241 (1): 291-296, 1996.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers chloramphenicol
Growth remarks For carotenoid production, e.g., 2YT medium including 30 mg/L of Cm at 20℃
Gene/insert name Pantoea ananatis crtE, crtB; Rhodobacter capsulatus Rc-crtI.
Reference of insert sequence LC643779 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Rhodobacter capsulatus crtI Pantoea ananatis crtB Pantoea ananatis crtE
      Reference sequence
        D90087.2, J04969.1
       

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takaichi, S. et al. Eur. J. Biochem. 241(1): 291-296, 1996)
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      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takaichi, S. et al. Eur. J. Biochem. 241(1): 291-296, 1996)。
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      RDB19485 pACCRT-EB-RcI Under QC test. Please contact us. DNA solution

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      How to cite this biological resource

      Materials & Methods section:

      The pACCRT-EB-RcI was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19485).

      Reference section:

      Takaichi, S., Sandmann, G., Schnurr, G., Satomi, Y., Suzuki, A., Misawa, N., The carotenoid 7,8-dihydro-psi end group can be cyclized by the lycopene cyclases from the bacterium Erwinia uredovora and the higher plant Capsicum annuum. Eur. J. Biochem. 241 (1): 291-296 (1996). PMID 8898919. [link to RRC of NBRP]

      Further references such as user reports and related articles (go to bottom)

      References and tips

      Featured content

      Featured content Biosynthesis of carotenoid (English text)

      QC test results

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References

      Original, user report and related articles

      original Takaichi, S., The carotenoid 7,8-dihydro-psi end group can be cyclized by the lycopene cyclases from the bacterium Erwinia uredovora and the higher plant Capsicum annuum. Eur. J. Biochem. 241 (1): 291-296 (1996). PMID 8898919. [link to RRC of NBRP]
      reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

      2023.06.27

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