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pAC-HIEBIA

pACYC184 vector-based plasmid for biosynthesis of γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, cruA).

Catalog number RDB19497
Resource name pAC-HIEBIA
Clone info. pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See STable 1 of Takemura, M. et al., Synth. Biol., 6 (1): ysab012, 2021.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI; Chlorobium phaeobacteroides cruA.
Reference of insert sequence LC654936 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank
External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Pantoea ananatis crtE Pantoea ananatis crtB Pantoea ananatis crtI Chlorobium phaeobacteroides cruA
Reference sequence
  AB019034, D90087.2
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)。
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RDB19497 pAC-HIEBIA Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pAC-HIEBIA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19497).

Reference section:

Takemura, M., Kubo, A., Watanabe, A., Sakuno, H., Minobe, Y., Sahara, T., Murata, M., Araki, M., Harada, H., Terada, Y., Yaoi, K., Ohdan, K., Misawa, N., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

Featured content Biosynthesis of carotenoid (English text)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

2023.06.27

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