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pAC-HIEBIA

pACYC184 vector-based plasmid for biosynthesis of γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli (HpIDI, crtE, crtB, crtI, cruA).

Catalog number RDB19497
Resource name pAC-HIEBIA
Clone info. pACYC184 vector (Tc resistance)-based plasmid for biosynthesis of γ-carotene (additionally including HpIDI) under the direction of the tac promoter in E. coli. See STable 1 of Takemura, M. et al., Synth. Biol., 6 (1): ysab012, 2021.
Comment Expression was confirmed by the depositor: formation of E. coli colonies with yellow pigmentation.
Vector backbone pACYC184 (plasmid)
Selectable markers tetracycline
Growth remarks For carotenoid production, e.g., 2YT medium including 12.5 mg/L of Tc and 0.05 mM IPTG at 20℃
Gene/insert name Haematococcus pluvialis HpIDI; Pantoea ananatis crtE, crtB, crtI; Chlorobium phaeobacteroides cruA.
Reference of insert sequence LC654936 (DDBJ accession number)
Depositor|Developer Misawa, Norihiko |
Other clones in our bank

External Database
Haematococcus pluvialis IDI (isopentenyl diphosphate isomerase); HpIDI Pantoea ananatis crtE Pantoea ananatis crtB Pantoea ananatis crtI Chlorobium phaeobacteroides cruA
Reference sequence
  AB019034, D90087.2
 
Sequence (full) RDB19497hts01.seqcheck assembled from experimentally sequenced data
Test sheet RDB19497_B3Iqp1-1.pdf 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)
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提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takemura, M. et al. Synth. Biol (Oxf). 6(1): ysab012, 2021)。
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RDB19497 pAC-HIEBIA DNA solution


How to cite this biological resource

Materials & Methods section:

The pAC-HIEBIA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19497).

Reference section:

Takemura, M., Kubo, A., Watanabe, A., Sakuno, H., Minobe, Y., Sahara, T., Murata, M., Araki, M., Harada, H., Terada, Y., Yaoi, K., Ohdan, K., Misawa, N., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


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Featured content

Featured content Biosynthesis of carotenoid (English text)

References

Original, user report and related articles

original Takemura, M., Pathway engineering for high-yield production of lutein in Escherichia coli. Synth. Biol. (Oxf) 6 (1): ysab012 (2021). PMID 34712837. [link to RRC of NBRP]
reference Misawa, N., Carotenoids: Carotenoid and apocarotenoid analysis-Use of E. coli to produce carotenoid standards. Methods Enzymol. 670: 87-137 (2022). PMID 35871847. [link to RRC of NBRP]

2024.02.15

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