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Akihiko Nakano's Resource

Akihiko Nakano's web page in English    in Japanese.

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List of S. cerevisiae vectors [link]

Catalog #Name of cloneRunning titlePublication
RDB08549pNY24-GLO3Plasmid clone for overproduction of GLO3 in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08550pNY24-3HA-GLO3Plasmid clone for overproduction of GLO3 in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08551pNY24-GCS1Plasmid clone for overproduction of GCS1 in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08552pNY24-GCS1-3HAPlasmid clone for overproduction of GCS1 in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08553pNY24-GLO3-A-GCS1Plasmid clone for overproduction of GLO3-GCS1 chimeric protein in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08554pNY24-GLO3-N-GCS1Plasmid clone for overproduction of GLO3-GCS1 chimeric protein in S. cerevisiae.Yahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08555pNY24-GCS1-A-GLO3Plasmid clone for overproduction of GCS1-GLO3 chimeric protein in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08556pNY24-GCS1-N-GLO3Plasmid clone for overproduction of GCS1-GLO3 chimeric protein in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08557pNY24-AGE1Plasmid clone for overproduction of AGE1 in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08558pNY24-AGE2Plasmid clone for overproduction of AGE2 in S. cerevisiaeYahara, N., The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J. Cell Sci. 119, 2604-2612 (2006). PMID 16735437. (original).
RDB08559pKU1Plasmid clone of TAT2Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08560pKU41Plasmid clone containing disruption copy of tat2 with ADE2Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08561pKU46 (YCpTAT2-3HA)Plasmid clone for overproduction of TAT2-3xHA in S. cerevisiaeUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08562pKU51Plasmid clone to express TAT-3xHA fusion protein in S. cerevisiaeUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08563pKU60Plasmid clone containing disruption copy of pep4 with HIS3.Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08564pKU62Plasmid clone containing disruption copy of vps1 with HIS3Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08565pKU65Plasmid clone containing disruption copy of vps27 with HIS3Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08566pKU68Plasmid clone for overproduction of TAT2 in S. cerevisiaeUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08567pKU76 (YCpTAT2-GFP)Plasmid clone for overproduction of TAT2-GFP in S. cerevisiaeUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08568pKU82Plasmid clone containing disruption copy of tat2 with hisG-URAUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08569pKU84Plasmid clone to express GFP-Pep12p fusion protein under the control of TDH3 promoter in S. cerevisiaeUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08570pKU105Expression clone of TRP1-Ub with CUP1 promoterUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08571pKU106Expression clone of TRP1-myc-Ub with CUP1 promoterUmebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08572pKU108Plasmid clone containing disruption copy of doa4 with HIS3.Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08573pKU121Plasmid clone containing disruption copy of bul1 with LEU2Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08574pKU131Plasmid clone containing disruption copy of erg13 with HIS3Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08575pKU158 (YCpTAT2^3K>R-3HA)Plasmid clone for overproduction of TAT2-3xHA in S. cerevisiae. 3KR mutant.Umebayashi, K., Ergosterol is required for targeting of tryptophan permease to the yeast plasma membrane. J. Cell Biol. 161,1117-1131 (2003). PMID 12810702. (original).
RDB08576pOTA1-1::ADE2 (YIpARF1)Plasmid clone for integration of ARF1 copy into the chromosomal ADE2 siteYahara, N., Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Mol. Biol. Cell. 12, 221-238 (2001). PMID 11160834. (original).
RDB08577arf1 deltaPlasmid clone containing disruption copy of ARF1::HIS3Yahara, N., Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Mol. Biol. Cell. 12, 221-238 (2001). PMID 11160834. (original).
RDB08578arf2 deltaPlasmid clone containing disruption copy of ARF2::HIS3Yahara, N., Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Mol. Biol. Cell. 12, 221-238 (2001). PMID 11160834. (original).
RDB08579pNY14-A1SPlasmid clone for overproduction of ARF1 in S. cerevisiaeYahara, N., Multiple roles of Arf1 GTPase in the yeast exocytic and endocytic pathways. Mol. Biol. Cell. 12, 221-238 (2001). PMID 11160834. (original).
RDB08580SEC12-MF(alpha)1 (pSKY1)Plasmid clone for overproduction of SEC12-MF(alpha)1 chimeric protein in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08581pRS316MS71HPlasmid clone to express MF(alpha)1-SEC71 chimeric protein tagged with 3xHA in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08582pYO326MS71HPlasmid clone for overproduction of MF(alpha)1-SEC71 chimeric protein tagged with 3xHA in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08583pRS316MS63FPlasmid clone to express MF(alpha)1-SEC63 chimeric protein tagged with myc in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08584pYO326MS63FPlasmid clone for overproduction of MF(alpha)1-SEC63 chimeric protein tagged with myc in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08585pSKY5wRER1-0Plasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08586pSKY5wRER1Y68LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08587pSKY5wRER1N76LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08588pSKY5wRER1Q77LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08589pSKY5wRER1T83LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08590pSKY5wRER1T128LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08591pSKY5wRER1S131LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08592pSKY5wRER1S135LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08593pSKY5wRER1S138LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08594pSKY5wRER1Y152LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08595pSKY5wRER1T160LPlasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08596pYO324/RER1-3HAPlasmid clone for overproduction of Rer1p tagged with 3xHA in S. cerevisiaeSato, K., Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. Mol. Biol. Cel. 6, 1459-1477 (1995). PMID 8589449. (original).
RDB08597pYO324/RER1-3HA Y152LPlasmid clone for overproduction of Rer1p tagged with 3xHA in S. cerevisiaeSato, K., Membrane protein retrieval from the Golgi apparatus to the endoplasmic reticulum (ER): characterization of the RER1 gene product as a component involved in ER localization of Sec12p. Mol. Biol. Cel. 6, 1459-1477 (1995). PMID 8589449. (original).
RDB08598pQR316-SEC71-GFPPlasmid clone to express SEC71 tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08599pQR326-SEC71-GFPPlasmid clone for overproduction of SEC71 tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08600pRS306-SEC71TMD-GFP/LSPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08601pRS306-SEC71TMD-GFP/SLPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08602pRS306-SEC71TMD-GFP/YLPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08603pRS306-SEC71TMD-GFP/1LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08604pRS306-SEC71TMD-GFP/2LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08605pRS306-SEC71TMD-GFP/3LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08606pRS306-SEC71TMD-GFP/4LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08607pRS306-SEC71TMD-GFP/2L delta LVPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08608pQR316-SEC71TMD-GFP/LSPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08609pQR316-SEC71TMD-GFP/SLPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08610pQR316-SEC71TMD-GFP/1LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08611pQR316-SEC71TMD-GFP/2LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08612pQR316-SEC71TMD-GFP/3LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08613pQR316-SEC71TMD-GFP/4LPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08614pQR316-SEC71TMD-GFP/2L delta LVPlasmid clone to express SEC71 transmembrane domain tagged with GFP in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. (original).
RDB08615pSKY5-RER1-0Plasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08616pSKY5-RER1-5Plasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08617pSKY5-RER1-10Plasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08618pSKY5-RER1-15Plasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08619pSKY5-RER1-20Plasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08620pSKY5-RER1-25Plasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08621pSKY5-RER1 G179APlasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endoplasmic reticulum membrane proteins, is dynamically localized to the Golgi apparatus by coatomer. J. Cell Biol. 152, 935-944 (2001). PMID 11238450. (original).
RDB08622pSKY5-RER1-K180SPlasmid clone to express GFP-Rer1p fusion protein under the control of TDH3 promoter in S. cerevisiaeSato, K., Rer1p, a retrieval receptor for endopla