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314-GNT1-GFP

Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae.

Catalog number RDB14671
Resource name 314-GNT1-GFP
Alternative name pRS314-ADH1p-GNT1-GFP-CMK1t
Clone info. Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae. ADH1 promoter and CMK1 terminator with the TRP1 marker.
Expression was confirmed by the depositor with microscopy observation.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name Saccharomyces cerevisiae GNT1 cDNA Aequorea victoria GFP cDNA
Depositor Nakano, Akihiko |
 
Other clones in our bank Saccharomyces cerevisiae GNT1 |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)
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Schedule A (GFP-A) [Link]
Remarks
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Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14671 314-GNT1-GFP Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311.

2019.07.19

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