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DSDm NQ-L

Plasmid clone to express DAP2 (cyt, lum)-SEC12 (tmd)-MF(alpha)1 chimeric protein in S. cerevisiae

Catalog number RDB08858
Resource name DSDm NQ-L
Alternative name 318
Clone info. Plasmid clone to express DAP2 (cyt, lum)-SEC12 (tmd)-MF(alpha)1 chimeric protein in S. cerevisiae. N358L, Q370L mutant. FFTNFILIVLLSYILQFSL to FFTLFILIVLLSYILLFSL.
Comment DAP2 (lum, cyt)-SEC12(tmd, NQ/L)-MF(alpha)1 fusion (DSD-NQ/L) on pSQ326 (2 micron, URA3)
Vector backbone pSQ326 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae DAP2 S. cerevisiae SEC12 S. cerevisiae MF(ALPHA)1
Depositor|Developer Nakano, Akihiko |
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External Database
S. cerevisiae DAP2 S. cerevisiae SEC12 S. cerevisiae MF(ALPHA)1

      Reference sequence
        
       
      Sequence RDB08858z.seq
      Remarks, protocol and/or map (pdf) RDB08858.pdf

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Cell Biol. 134, 279-293, 1996).
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      提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Cell Biol. 134, 279-293, 1996)。
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      RDB08858 DSDm NQ-L Under QC test. Please contact us. DNA solution

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      How to cite this biological resource

      Materials & Methods section:

      The DSDm NQ-L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08858).

      Reference section:

      Sato, M., Sato, K., Nakano, A., Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain. J. Cell Biol. 134, 279-293 (1996). PMID 8707815. [link to RRC of NBRP]

      Further references such as user reports and related articles (go to bottom)


      References and tips

      Featured content

      Featured content Akihiko Nakano's S. cerevisiae clones (English text)

      QC test results

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


      References

      Original, user report and related articles

      original Sato, M., Endoplasmic reticulum localization of Sec12p is achieved by two mechanisms: Rer1p-dependent retrieval that requires the transmembrane domain and Rer1p-independent retention that involves the cytoplasmic domain. J. Cell Biol. 134, 279-293 (1996). PMID 8707815. [link to RRC of NBRP]

      2023.06.19

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