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316-GNT1-GFP (#RDB14670)

Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae.

Alternative name pRS316-ADH1p-GNT1-GFP-CMK1t
Clone info. Expression vector of S. cerevisiae GNT1, fused with GFP for labeling medial cisternae. ADH1 promoter and CMK1 terminator with the URA3 marker, low-copy plasmids.
Comment Expression was confirmed by the depositor with microscopy observation.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name Saccharomyces cerevisiae GNT1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Nakano, Akihiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14670 316-GNT1-GFP Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The 316-GNT1-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14670).

Reference section:

Ishii, M., Suda, Y., Kurokawa, K., Nakano, A., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311. [link to RRC of NBRP]