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316-MW71WS/4L

Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein in S. cerevisiae

Catalog number RDB08641
Resource name 316-MW71WS/4L
Alternative name 97
Clone info. Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein tagged with 3xHA under the control of SEC63 promoter in S. cerevisiae. 4L mutation in the transmembrane domain of Sec71p.
MW71WS-4L mutant on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae WBP1 S. cerevisiae Sec71 S. cerevisiae MF(ALPHA)1
Depositor Nakano, Akihiko |
 
Other clones in our bank S. cerevisiae WBP1 (KEGG orthology K12670) | S. cerevisiae SEC66 (KEGG orthology K12273) | S. cerevisiae MF(ALPHA)1 (KEGG orthology K11234) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003).
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RDB08641 316-MW71WS/4L DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08641z.seq from Depositor
Electronic file PDF RDB08641.pdf from Depositor

Featured content

Featured content Akihiko Nakano's S. cerevisiae clones (English text)

References

original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550.

2018.12.10

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