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pKSE122 (#RDB08703)

Expression clone of S. cerevisiae Emp47p C-ternimal tail fused with GST in E. coli

Alternative name 159
Clone info. Expression clone of S. cerevisiae Emp47p C-ternimal tail fused with GST in E. coli. C-terminal mutation KTKLL to STSLL.
Comment GST-EMP47Ct (STSLL)
Vector backbone pGEX4t-1 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae GST-EMP47Ct(STSLL)
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08703z.seq
Remarks, protocol and/or map (pdf) RDB08703.pdf

Distribution information

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Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Sato, K. and Nakano, A., Mol. Biol. Cell 13, 2518-2532, 2002).
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必要書類
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提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Sato, K. and Nakano, A., Mol. Biol. Cell 13, 2518-2532, 2002)。

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RDB08703 pKSE122 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pKSE122 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08703).

Reference section:

Sato, K., Nakano, A., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Cell 13, 2518-2532 (2002). PMID 12134087. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Sato, K., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Cell 13, 2518-2532 (2002). PMID 12134087. [link to RRC of NBRP]