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pYS2-2B

Plasmid clone containing disruption copy of HRD3 (EKS1)

Catalog number RDB08777
Resource name pYS2-2B
Alternative name 234
Clone info. Plasmid clone containing disruption copy of HRD3 (EKS1), constructed from pYS1-6 clone.
Comment hrd3::TRP1 on pBluescript II SK+
Vector backbone pBluescript II SK+ (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae hrd3::TRP1
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae hrd3::TRP1

          Reference sequence
            
           
          Sequence RDB08777z.seq
          Remarks, protocol and/or map (pdf) RDB08777.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 125, 130-137, 1999).
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          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem. 125, 130-137, 1999)。
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          提供依頼書 [Word]
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          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB08777 pYS2-2B Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pYS2-2B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08777).

          Reference section:

          Saito, Y., Yamanushi, T., Oka, T., Nakano, A., Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase. J. Biochem. 125, 130-137 (1999). PMID 9880808. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Saito, Y., Identification of SEC12, SED4, truncated SEC16, and EKS1/HRD3 as multicopy suppressors of ts mutants of Sar1 GTPase. J. Biochem. 125, 130-137 (1999). PMID 9880808. [link to RRC of NBRP]

          2023.06.19

          GNP_filter3_RDBDEP_html_230615.pl