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316-M71WWS

Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein in S. cerevisiae

Catalog number RDB08630
Resource name 316-M71WWS
Alternative name 86
Clone info. Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein tagged with 3xHA under the control of SEC63 promoter in S. cerevisiae. SSTN substitution.
MF(alpha)1-3HA-SEC71(lum)-Wbp1(tmd)-Wbp1(cyt, SSTN) (SEC63 promoter) on pRS316
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SEC71 S. cerevisiae WBP1 S. cerevisiae MF(ALPHA)1
Depositor Nakano, Akihiko |
 
Other clones in our bank S. cerevisiae SEC66 (KEGG orthology K12273) | S. cerevisiae WBP1 (KEGG orthology K12670) | S. cerevisiae MF(ALPHA)1 (KEGG orthology K11234) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003).
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RDB08630 316-M71WWS DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08630z.seq from Depositor
Electronic file PDF RDB08630.pdf from Depositor

Featured content

Featured content Akihiko Nakano's S. cerevisiae clones (English text)

References

original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550.

User reports and related articles (go to bottom)


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

References

original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550.

2018.12.10

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