Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pSKY5wRER1-0 (#RDB08585)

Plasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiae

Alternative name 41
Clone info. Plasmid clone to express RER1 protein tagged with GFP under the control of TDH3 promoter in S. cerevisiae.
Comment GFP-RER1 (TDH3 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae RER1
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08585z.seq
Remarks, protocol and/or map (pdf) RDB08585.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Cell. 14, 3605-3616, 2003)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08585 pSKY5wRER1-0 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pSKY5wRER1-0 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08585).

Reference section:

Sato, K., Sato, M., Nakano, A. , Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08585_A5D1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: TRP1pro_F
Sequence file: RDB08585_A5D1a.seq check
>08585_08585_A5D1_TRP1pro_F_F01_16.ab1
    1 GCAAGGTGAA TAGCAACAAG GCAGCTTGGA GTATGTCTGT TATTAATTTC ACAGGTAGTT
   61 CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACGGA GGCCGCAGAA TGTGCTCTAG
  121 ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA ACAATTGACC
  181 CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT TCAGGCACTC
  241 CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG GCTCTGGTCA
  301 ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA GAATACCAAG
  361 AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC AACATACTAC
  421 TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA GAAGCAGGTG
  481 GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG CAAGAGAGCC
  541 CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT GGTGATGCGC
  601 TTAGATTAAA TGGCGTTATT GGTGTTGATG TAAGCGGAGG TGTGGAGACA AATGGTGTAA
  661 AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT ATTACTGAGT
  721 AGTATTTATT TAAGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA ATACCGCACA
  781 GATGCGTAAG GAGAAAATAC CGCATCAGGA AATTGTAAGC GTTAATATTT TGTTAAAATT
  841 CGCGTTAAAT TTTTGTTAAA TCAGCTCATT TTTTAACCAA TAGGCCGAAA TCGGCAAAAT
  901 CCCTTATAAA TCAAAAGAAT AGACCGAGAT AGGGTTGAGT GTTGTTCCAG TTTGGAACAA
  961 GAGTCCACTA TTAAAGAACG TGGACTCCAA CGTCAAAAGG GCGAAAAACC CGTCTATCAG
 1021 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAGTTTT TTTGGGGTCG AGTGCCGTAA
 1081 AGCACTTAAT TCGACCTTAA TGGAGCCCCC GATTTAAGCT GACTGGCGAG CGGCGAACGT
1141 GCGAGAACGC AAGGCAAGAA AAAG
//
Primer: TDH3pro_F
Sequence file: RDB08585_A5D1b.seq check
>08585_08585_A5D1_TDH3pro_F_G01_19.ab1
    1 TAGCCCTGTA GGTATTGATT GTAATTCTGT AAATCTATTT CTTAAACTTC TTAAATTCTA
   61 CTTTTATAGT TAGTCTTTTT TTTAGTTTTA AAACACCAAG AACTTAGTTT CGAATAAACA
  121 CACATAAACA GAATTCGCGG CCGCAGATCC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC
  181 GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG
  241 TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
  301 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG
  361 TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC
  421 GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC
  481 GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC
  541 TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
  601 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC
  661 AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC
  721 GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA
  781 GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC
  841 ACTCTCGGCA TGGACGAGCT GTACAAGAGA TCTATGGATT ACGATAGCTC TGATACAATG
  901 AACGGTGGTT CAAGTAACCC CTTAATCACT AAGATGAATA CAATGAAATT ATTATATCAA
  961 CACTATTTGG ATAAAGTCAC TCCTCACGCT AAAGGAGAGG TGGGCTGTAT TGGTGGGTTT
 1021 GTTATGTTTG TTTATGGTTT CGTATTACAA TGGCCCGAAC GCTGGGTATG TGATTTGCTA
 1081 TGGGTCTAGT CTATTTTTTA TGGATTCACT TTTTAAGACG TTTTTTGACC CCAAAATTCG
1141 ATATATTGTT TCCGTTTACG
//
Primer: EGFP-C
Sequence file: RDB08585_A5D1c.seq check
>08585_08585_A5D1_EGFP-C_H01_22.ab1
    1 AAGGGATATC TCGGGCATGG ACGAGCTGTA CAAGAGATCT ATGGATTACG ATAGCTCTGA
   61 TACAATGAAC GGTGGTTCAA GTAACCCCTT AATCACTAAG ATGAATACAA TGAAATTATT
  121 ATATCAACAC TATTTGGATA AAGTCACTCC TCACGCTAAG GAGAGGTGGG CTGTATTGGG
  181 TGGTTTGTTA TGTTTGTTTA TGGTTCGTAT TACAATGGCC GAAGGCTGGT ATGTGATTTG
  241 TTATGGTCTA GGTCTATTTT TATTGAATCA ATTTTTAGCC TTTTTGACCC CAAAATTCGA
  301 TATGTCCTTA CAGCAAGATG AAGAAAACAA CGAATTGGAA GCTGGAGAAA AATCAGAAGA
  361 ATTCCGTCCA TTCATCAGAA GATTACCAGA GTTCAAATTC TGGTATAACA GCATTAGAGC
  421 CACTGTCATT TCCCTCTTGT TGTCGCTATT TTCAATCTTC GATATTCCAG TATTTTGGCC
  481 CATCTTATTG ATGTATTTCA TATTATTGTT TTTTTTAACT ATGAGAAGGC AGATTCAACA
  541 TATGATAAAA TATAGATATA TACCCTTAGA TATCGGTAAG AAGAAATATT CTCATTCTTC
  601 TAACTGAAGC TTTTGCACAA TTAGTTAACA CAGTGCTTGC CGAAAAGGAA AAATTTTTAA
  661 ATATTAACAG AGTCTGTAGT TCTGATAGCG ATCTTCCTGG GAGTGATATA AAGTCTTTGG
  721 ATGAGGCGAA GGAGAAACCA GAGGGGAAGG ATACCAAAAC AGAAGAATGA TAACAGTATG
  781 CCGTTTTACC ACACTGGCGA ATAAATCTTC AATGACTTTG TAAGCTCGAA TATGCGCAAG
  841 ATTTGACTTT GATATTTTGC ACTTTTTCGT CCTCACTTTC TACCTTTTTT TTTTTATGCC
  901 TTAGTTGAAA AGATACGCGT ATCTAATTAT GTACTAATAG TCTTAATTTT ACATTACTTA
  961 ATTAATCGAA ATGCAGCCTG TAAGGAGTTT CACAATCTTT CACGTTCGTT GAAAGGCTAG
 1021 TTGTCTAAGT GCGCTGCCCT TTTAACTCTA CCATCATCAA AAGAAGAATC ATTGTCGTGG
 1081 TCAATCATAA TATCAGCTTC GTCGTCTCGT CATCATCGAA CAATATTTCC TCGCTATCAT
1141 GTCGCAAATT ACGTTACGTT CTGTTCGCGA TAAATC
//
Primer: Amp_R
Sequence file: RDB08585_A5D1d.seq check
>08585_08585_A5D1_Amp_R_A02_02.ab1
    1 TTCGGTTTTA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA
  181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC
  241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC
  301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA
  361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT
  421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT
  481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC
  541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA
  601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC
  661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT
  721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA
  781 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG
  841 GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA TAGATCACGA
  901 CATTACTATA TATATAATAT AGGAAGCATT TAATAGAACA GCATCGTAAT ATATGTGTAC
  961 TTTGCAGTTA TGACGCCAGA TGGCAGTAGT GGAAAGATAT TCTTTATTGA AAAATAGCTT
 1021 GTCACCTTTA CGTACAATCT TGATCCGGGA GCTTTTCTTT TTTTGCCGAT AGAATTATTC
 1081 GGTCGAAAAA AAGAAAATGA GAGGCCAGAG GAGGCATGTG ACTATGAGCA CGTGAGTATA
1141 CGTGATTAGC ACACAAAGGC CAGCCTTGGG GAGATAGG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP]