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316-SYS1-GFP

Expression vector of S. cerevisiae SYS1, fused with GFP for labeling trans cisternae.

Catalog number RDB14672
Resource name 316-SYS1-GFP
Alternative name pRS316-ADH1p-SYS1-GFP-CMK1t
Clone info. Expression vector of S. cerevisiae SYS1, fused with GFP for labeling trans cisternae. ADH1 promoter and CMK1 terminator with the URA3 marker, low-copy plasmids.
Expression was confirmed by the depositor with microscopy observation.
Vector backbone pRS316 (plasmid)
Selectable markers Amp^r (E. coli), URA3
Gene/insert name Saccharomyces cerevisiae SYS1 cDNA Aequorea victoria GFP cDNA
Depositor Nakano, Akihiko |
 
Other clones in our bank Saccharomyces cerevisiae SYS1 (KEGG orthology K20318) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)
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Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14672 316-SYS1-GFP Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311.

2019.07.19

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