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pTU1-PalphaAPP

Plasmid clone to express PalphaAPP chimeric protein in S. cerevisiae

Catalog number RDB08714
Resource name pTU1-PalphaAPP
Alternative name 170
Clone info. Plasmid clone to express PalphaAPP chimeric protein under the control of TDH3 promoter in S. cerevisiae.
Comment MF(alpha)1-APP1(human beta-amyloid precursor protein) fusion (TDH3 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name human beta-amyloid precursor protein (APP) S. cerevisiae MF(ALPHA)1
Depositor|Developer Nakano, Akihiko |
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External Database
human beta-amyloid precursor protein (APP) S. cerevisiae MF(ALPHA)1

        Reference sequence
          
         
        Sequence RDB08714z.seq
        Remarks, protocol and/or map (pdf) RDB08714.pdf

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Genes to Cells. 14, 205-215, 2009).
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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        提供案内 (日本国内) [open/close]

        提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Genes to Cells. 14, 205-215, 2009)。
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        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB08714 pTU1-PalphaAPP Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        How to cite this biological resource

        Materials & Methods section:

        The pTU1-PalphaAPP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08714).

        Reference section:

        Murakami-Sekimata, A., Sato, K., Sato, K., Takashima, A., Nakano, A., O-Mannosylation is required for the solubilization of heterologously expressed human beta-amyloid precursor protein in Saccharomyces cerevisiae. Genes to Cells. 14, 205?215 (2009). PMID 19170767. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


        References and tips

        Featured content

        Featured content Akihiko Nakano's S. cerevisiae clones (English text)

        QC test results

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


        References

        Original, user report and related articles

        original Murakami-Sekimata, A., O-Mannosylation is required for the solubilization of heterologously expressed human beta-amyloid precursor protein in Saccharomyces cerevisiae. Genes to Cells. 14, 205?215 (2009). PMID 19170767. [link to RRC of NBRP]

        2023.06.19

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