Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein in S. cerevisiae
Catalog number | RDB08639 |
---|---|
Resource name | 316-MW71WS/2L |
Alternative name | 95 |
Clone info. | Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein tagged with 3xHA under the control of SEC63 promoter in S. cerevisiae. 2L mutation in the transmembrane domain of Sec71p. MW71WS-2L mutant on pRS316 (pCEN, URA3) |
Vector backbone | pRS316 (Plasmid) |
Selectable markers | Amp |
Gene/insert name | S. cerevisiae WBP1 S. cerevisiae Sec71 S. cerevisiae MF(ALPHA)1 |
Depositor | Nakano, Akihiko | |
  | |
Other clones in our bank | S. cerevisiae WBP1 (KEGG orthology K12670) | S. cerevisiae SEC66 (KEGG orthology K12273) | S. cerevisiae MF(ALPHA)1 (KEGG orthology K11234) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR | Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003). |
---|---|
Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB08639 | 316-MW71WS/2L | DNA solution |
Electronic file
Electronic file | Sequence RDB08639z.seq from Depositor |
---|---|
Electronic file | PDF RDB08639.pdf from Depositor |
Featured content
Featured content | Akihiko Nakano's S. cerevisiae clones (English text) |
---|
References
original | Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. |
---|
User reports and related articles (go to bottom)
This clone will be sequenced a portion and digested by restriction enzyme for examination.
References
original | Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. |
---|
2018.12.10
GNP_filter3_RDBDEP_html_181012.pl