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316-MW71WS/2L

Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein in S. cerevisiae

Catalog number RDB08639
Resource name 316-MW71WS/2L
Alternative name 95
Clone info. Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein tagged with 3xHA under the control of SEC63 promoter in S. cerevisiae. 2L mutation in the transmembrane domain of Sec71p.
Comment MW71WS-2L mutant on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae WBP1 S. cerevisiae Sec71 S. cerevisiae MF(ALPHA)1
Depositor|Developer Nakano, Akihiko |
Other clones in our bank
External Database
S. cerevisiae WBP1 S. cerevisiae Sec71 S. cerevisiae MF(ALPHA)1
      Reference sequence
        
       
      Sequence RDB08639z.seq
      Remarks, protocol and/or map (pdf) RDB08639.pdf

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003).
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      提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Cell. 14, 3605-3616, 2003)。
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      RDB08639 316-MW71WS/2L Under QC test. Please contact us. DNA solution

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      How to cite this biological resource

      Materials & Methods section:

      The 316-MW71WS/2L was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08639).

      Reference section:

      Sato, K., Sato, M., Nakano, A. , Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP]

      Further references such as user reports and related articles (go to bottom)

      References and tips

      Featured content

      Featured content Akihiko Nakano's S. cerevisiae clones (English text)

      QC test results

      check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

      References

      Original, user report and related articles

      original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP]

      2023.06.19

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