Resource data sheet
Prev.

304-SEC71TMD-GFP

Expression vector of S. cerevisiae SEC71TMD (Sec71_transmembrane domain), fused with GFP for labeling ER.

Catalog number RDB14673
Resource name 304-SEC71TMD-GFP
Alternative name pRS304-SEC71p-SEC71TMD-GFP-SEC71t
Clone info. Expression vector of S. cerevisiae SEC71TMD (Sec71_transmembrane domain), fused with GFP for labeling ER. SEC71 promoter and SEC71 terminator with the TRP1 marker.
Expression was confirmed by the depositor with microscopy observation.
Vector backbone pRS304 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name Saccharomyces cerevisiae SEC66 (SEC71) cDNA Aequorea victoria GFP cDNA
Depositor Nakano, Akihiko |
 
Other clones in our bank Saccharomyces cerevisiae SEC66 (KEGG orthology K12273) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks
Please use Schedule A (GFP-A) additionally.
Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14673 304-SEC71TMD-GFP Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311.

2019.07.19

GNP_filter3_RDBDEP_html_190432.pl