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304-SEC71TMD-GFP (#RDB14673)

Expression vector of S. cerevisiae SEC71TMD (Sec71_transmembrane domain), fused with GFP for labeling ER.

Alternative name pRS304-SEC71p-SEC71TMD-GFP-SEC71t
Clone info. Expression vector of S. cerevisiae SEC71TMD (Sec71_transmembrane domain), fused with GFP for labeling ER. SEC71 promoter and SEC71 terminator with the TRP1 marker.
Comment Expression was confirmed by the depositor with microscopy observation.
Vector backbone pRS304 (plasmid)
Size of vector backbone 4.3 kb
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name Saccharomyces cerevisiae SEC66 (SEC71) cDNA Aequorea victoria GFP cDNA
Depositor|Developer Nakano, Akihiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishii, M. et al. J. Cell. Sci. 129(17), 3251-3261, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14673 304-SEC71TMD-GFP Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The 304-SEC71TMD-GFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14673).

Reference section:

Ishii, M., Suda, Y., Kurokawa, K., Nakano, A., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Ishii, M., COPI is essential for Golgi cisternal maturation and dynamics. J. Cell Sci. 129 (17): 3251-3261 (2016). PMID 27445311. [link to RRC of NBRP]