Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKSY105

Plasmid clone to express EMP47 tagged with 2xMyc in S. cerevisiae

Catalog number RDB08667
Resource name pKSY105
Alternative name 123
Clone info. Plasmid clone to express EMP47 tagged with 2xMyc in S. cerevisiae.
Comment 2myc-EMP47 on pRS314 (pCEN, TRP1)
Vector backbone pRS314 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae EMP47
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae EMP47

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Celll.13, 2518-2532, 2002).
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Celll.13, 2518-2532, 2002)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08667 pKSY105 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08667z.seq
          Remarks, protocol and/or map (pdf) RDB08667.pdf

          Original reference

          original Sato, K., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Celll.13, 2518-2532 (2002). PMID 12134087.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08667_A4Ii.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB08667_A4Iia.seq check
          >08667_08667_A4Ii_T7_20bp_F09_18.ab1
              1 TTCCACTTCG CGGGGGGGCG GGCCGCGTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA
             61 TTCCCCTCAG GGCCCTCGAA ACTGTCACCC CTTCGTCTTC ATCTTCCTGC ACTGCCTTCA
            121 GGTTGTCCAT ACCGGCTTCT ATACCAAACA ACTGCCTCCC ATGACTGACT TCAAATTGCA
            181 TTCTTGACGC AAATAATTGA ATGAACAAGT CGCATTGCTC ACAACCATGG TATAATCTAT
            241 CAAAAAGCAC ATCTAGACCT GAGACAAGGT CTTCAGACCA AAAATAGTCG CAAAATGATA
            301 GCATGCTCGC TTGCAGGTGT ACAACATCTA TTACAATTCA ACGGAATATT ATTCCTGTTT
            361 CATAATATTT CAATTTCCAT CTATTAATGT CATTACGTCT TGCTATCACT ATACACGAGT
            421 AGAGAAACAG TATGAACTCT TAAAAATGTT TTTGATCTTT TAAGAGAAGC CTTAATTGGT
            481 ATTGAATAAT TAGAATTATA CCAATAAAAT TAACAAATAG TTAGCATCAG GGAATCTGTA
            541 TTTTATGATG ATGTTAATTA CTATGAAAAG TACAGTACTG TTGAGTGTTT TTACCGTCTT
            601 ACCGACATGG GCTGGATTGC TAGAAGCTCA TCCATTGGGT GACACTTCAG ATGCAGAACA
            661 AAAGTTGATT TCTGAAGAAG ACTTGAACGA TGCATCCGAA CAAAATTGAT TTCTGAAGAT
            721 GATCTGAACG ATGCATCCAA ATTATGCTCA GACTACTCGC TCCCTGATCT CATTAATACA
            781 CGTAAAGTGC CCATTAACTG GCAAACTGGA GAACAAGCTA GTCTAGAGGA AGGGGAGAAT
            841 TGTATTGACT TCTAATCAAA TTTCCCAGGG TTCATTTTGG TTGAAGCAAG GATTCTATTT
          901 GAAGGATTCT TTTCTATGGG ATGGGACATT TAGGAGTGTT GGTTATTCTG GCC
          //
          Primer: Reverse2
          Sequence file: RDB08667_A4Iib.seq check
          >08667_08667_A4Ii_Reverse2_G09_21.ab1
              1 GGAGGGGCAC ATCAGGCTGC AGCGATTCGC CAGCTCGAAT TAACCCTCAC TAAAGGGAAC
             61 AAAAGCTGGT ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT
            121 CAAAAATAAA TATTTGAAAG ATGTGTTGGC GCAAACGAGT GTTACTACAA ATTTTAACGA
            181 AATAACGAAT ACGGATGAAA CAATAACAAC AAAGAGGGTA CCGAACCCAA ACTTCCTAGG
            241 AAATTGTAAC ATTAAGGATT TTAAACCTCC ATACATTTAT TCTCATGTGA ACAAAGTACC
            301 ACAAAATGTC GCTGGGGATA AAACGGCTGT TAAGCTGGAC ACTGAAGTAA AGAACACAAA
            361 TTCTAATCCA GTGGTGGCGA CGGATCCGGT CGCCGCTAAA CCGGACAACC TGGCTAACTT
            421 CAGCAACGAA GTAGCTATGA ATAATTGAAA GAAGCAAAAC ATAATGTAGG GAACACTCCA
            481 ATAAGAAATA AACCATTGTT GAAGTAGTAA TATAAACTAT CGTTCGTTAT TTTATAAAAT
            541 ATGTATTGTT ACATATATAT ACATATATAG GGAAGTGCAC AATTGGTTTG AGTGGAAGGA
            601 ATAATCCAAA ATCATAGCAG TTTGGTCTTT ATGATCTCTT GTCTGATTCT GAATGTGTAA
            661 TATGCCATAA CCAACATGAT GAAAATCAAT GGAAGTAACC AAATCATTAG TTTTCTGGCA
            721 ATTTCGTCGA CCTGAGGACC ATCTTGTCCA TGACGTTTGG TAGCTTCCCT ATACTTTTCT
            781 TGTTCAACCA GGACTTTCTC CAATTTCTGA TTGATTGATA AGAAGTCTTT GAAATTTTCT
            841 TTATCCTCAG CGATTGCATC ATCGGTGGAC GTTCCCTTTT CATTATTAGC TGGTGGCTTA
            901 GAAGAGAGCT GTTTAGTTAT CGTAGTCATG AATGATAATA GCTCTTGTTG GACCTTAATG
            961 ACATCATTTA GCTTTGTTTC TAAAGCATTG ATATCGTTCG CAGGAATTTT ACCTTCAACT
           1021 CTATCCAGTT TCTTATATAA ATTCATAGGT TTGTAATTTT GTCTTTGTCA GCGTCAAATG
           1081 CTGTTTTTTT TCCAATCATT TTTTCCTTGC GGTTTGTGGT TCAATGGAAT TGGTTGATCA
          1141 GCTTTGGCCT GACATTGGCA ATCCACATTA AGGGGGAATA TCTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sato, K., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Celll.13, 2518-2532 (2002). PMID 12134087.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl