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pKSY105

Plasmid clone to express EMP47 tagged with 2xMyc in S. cerevisiae

Catalog number RDB08667
Resource name pKSY105
Alternative name 123
Clone info. Plasmid clone to express EMP47 tagged with 2xMyc in S. cerevisiae.
2myc-EMP47 on pRS314 (pCEN, TRP1)
Vector backbone pRS314 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae EMP47
Depositor Nakano, Akihiko |
 
Other clones in our bank S. cerevisiae EMP47 (KEGG orthology K10080) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Celll.13, 2518-2532, 2002).
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RDB08667 pKSY105 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB08667z.seq from Depositor
Electronic file PDF RDB08667.pdf from Depositor

Featured content

Featured content Akihiko Nakano's S. cerevisiae clones (English text)

References

original Sato, K., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Celll.13, 2518-2532 (2002). PMID 12134087.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB08667_A4Ii.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB08667_A4Iia.seq
>08667_08667_A4Ii_T7_20bp_F09_18.ab1
    1 TTCCACTTCG CGGGGGGGCG GGCCGCGTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA
   61 TTCCCCTCAG GGCCCTCGAA ACTGTCACCC CTTCGTCTTC ATCTTCCTGC ACTGCCTTCA
  121 GGTTGTCCAT ACCGGCTTCT ATACCAAACA ACTGCCTCCC ATGACTGACT TCAAATTGCA
  181 TTCTTGACGC AAATAATTGA ATGAACAAGT CGCATTGCTC ACAACCATGG TATAATCTAT
  241 CAAAAAGCAC ATCTAGACCT GAGACAAGGT CTTCAGACCA AAAATAGTCG CAAAATGATA
  301 GCATGCTCGC TTGCAGGTGT ACAACATCTA TTACAATTCA ACGGAATATT ATTCCTGTTT
  361 CATAATATTT CAATTTCCAT CTATTAATGT CATTACGTCT TGCTATCACT ATACACGAGT
  421 AGAGAAACAG TATGAACTCT TAAAAATGTT TTTGATCTTT TAAGAGAAGC CTTAATTGGT
  481 ATTGAATAAT TAGAATTATA CCAATAAAAT TAACAAATAG TTAGCATCAG GGAATCTGTA
  541 TTTTATGATG ATGTTAATTA CTATGAAAAG TACAGTACTG TTGAGTGTTT TTACCGTCTT
  601 ACCGACATGG GCTGGATTGC TAGAAGCTCA TCCATTGGGT GACACTTCAG ATGCAGAACA
  661 AAAGTTGATT TCTGAAGAAG ACTTGAACGA TGCATCCGAA CAAAATTGAT TTCTGAAGAT
  721 GATCTGAACG ATGCATCCAA ATTATGCTCA GACTACTCGC TCCCTGATCT CATTAATACA
  781 CGTAAAGTGC CCATTAACTG GCAAACTGGA GAACAAGCTA GTCTAGAGGA AGGGGAGAAT
  841 TGTATTGACT TCTAATCAAA TTTCCCAGGG TTCATTTTGG TTGAAGCAAG GATTCTATTT
901 GAAGGATTCT TTTCTATGGG ATGGGACATT TAGGAGTGTT GGTTATTCTG GCC
//
Primer: Reverse2 RDB08667_A4Iib.seq
>08667_08667_A4Ii_Reverse2_G09_21.ab1
    1 GGAGGGGCAC ATCAGGCTGC AGCGATTCGC CAGCTCGAAT TAACCCTCAC TAAAGGGAAC
   61 AAAAGCTGGT ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT
  121 CAAAAATAAA TATTTGAAAG ATGTGTTGGC GCAAACGAGT GTTACTACAA ATTTTAACGA
  181 AATAACGAAT ACGGATGAAA CAATAACAAC AAAGAGGGTA CCGAACCCAA ACTTCCTAGG
  241 AAATTGTAAC ATTAAGGATT TTAAACCTCC ATACATTTAT TCTCATGTGA ACAAAGTACC
  301 ACAAAATGTC GCTGGGGATA AAACGGCTGT TAAGCTGGAC ACTGAAGTAA AGAACACAAA
  361 TTCTAATCCA GTGGTGGCGA CGGATCCGGT CGCCGCTAAA CCGGACAACC TGGCTAACTT
  421 CAGCAACGAA GTAGCTATGA ATAATTGAAA GAAGCAAAAC ATAATGTAGG GAACACTCCA
  481 ATAAGAAATA AACCATTGTT GAAGTAGTAA TATAAACTAT CGTTCGTTAT TTTATAAAAT
  541 ATGTATTGTT ACATATATAT ACATATATAG GGAAGTGCAC AATTGGTTTG AGTGGAAGGA
  601 ATAATCCAAA ATCATAGCAG TTTGGTCTTT ATGATCTCTT GTCTGATTCT GAATGTGTAA
  661 TATGCCATAA CCAACATGAT GAAAATCAAT GGAAGTAACC AAATCATTAG TTTTCTGGCA
  721 ATTTCGTCGA CCTGAGGACC ATCTTGTCCA TGACGTTTGG TAGCTTCCCT ATACTTTTCT
  781 TGTTCAACCA GGACTTTCTC CAATTTCTGA TTGATTGATA AGAAGTCTTT GAAATTTTCT
  841 TTATCCTCAG CGATTGCATC ATCGGTGGAC GTTCCCTTTT CATTATTAGC TGGTGGCTTA
  901 GAAGAGAGCT GTTTAGTTAT CGTAGTCATG AATGATAATA GCTCTTGTTG GACCTTAATG
  961 ACATCATTTA GCTTTGTTTC TAAAGCATTG ATATCGTTCG CAGGAATTTT ACCTTCAACT
 1021 CTATCCAGTT TCTTATATAA ATTCATAGGT TTGTAATTTT GTCTTTGTCA GCGTCAAATG
 1081 CTGTTTTTTT TCCAATCATT TTTTCCTTGC GGTTTGTGGT TCAATGGAAT TGGTTGATCA
1141 GCTTTGGCCT GACATTGGCA ATCCACATTA AGGGGGAATA TCTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Sato, K., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Celll.13, 2518-2532 (2002). PMID 12134087.

2018.12.10

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