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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKSY105 (#RDB08667)

Plasmid clone to express EMP47 tagged with 2xMyc in S. cerevisiae

Alternative name 123
Clone info. Plasmid clone to express EMP47 tagged with 2xMyc in S. cerevisiae.
Comment 2myc-EMP47 on pRS314 (pCEN, TRP1)
Vector backbone pRS314 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae EMP47
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08667z.seq
Remarks, protocol and/or map (pdf) RDB08667.pdf

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Sato, K. and Nakano, A., Mol. Biol. Cell 13, 2518-2532, 2002).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Sato, K. and Nakano, A., Mol. Biol. Cell 13, 2518-2532, 2002)。

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RDB08667 pKSY105 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pKSY105 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08667).

Reference section:

Sato, K., Nakano, A., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Cell 13, 2518-2532 (2002). PMID 12134087. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08667_A4Ii.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB08667_A4Iia.seq check
>08667_08667_A4Ii_T7_20bp_F09_18.ab1
    1 TTCCACTTCG CGGGGGGGCG GGCCGCGTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA
   61 TTCCCCTCAG GGCCCTCGAA ACTGTCACCC CTTCGTCTTC ATCTTCCTGC ACTGCCTTCA
  121 GGTTGTCCAT ACCGGCTTCT ATACCAAACA ACTGCCTCCC ATGACTGACT TCAAATTGCA
  181 TTCTTGACGC AAATAATTGA ATGAACAAGT CGCATTGCTC ACAACCATGG TATAATCTAT
  241 CAAAAAGCAC ATCTAGACCT GAGACAAGGT CTTCAGACCA AAAATAGTCG CAAAATGATA
  301 GCATGCTCGC TTGCAGGTGT ACAACATCTA TTACAATTCA ACGGAATATT ATTCCTGTTT
  361 CATAATATTT CAATTTCCAT CTATTAATGT CATTACGTCT TGCTATCACT ATACACGAGT
  421 AGAGAAACAG TATGAACTCT TAAAAATGTT TTTGATCTTT TAAGAGAAGC CTTAATTGGT
  481 ATTGAATAAT TAGAATTATA CCAATAAAAT TAACAAATAG TTAGCATCAG GGAATCTGTA
  541 TTTTATGATG ATGTTAATTA CTATGAAAAG TACAGTACTG TTGAGTGTTT TTACCGTCTT
  601 ACCGACATGG GCTGGATTGC TAGAAGCTCA TCCATTGGGT GACACTTCAG ATGCAGAACA
  661 AAAGTTGATT TCTGAAGAAG ACTTGAACGA TGCATCCGAA CAAAATTGAT TTCTGAAGAT
  721 GATCTGAACG ATGCATCCAA ATTATGCTCA GACTACTCGC TCCCTGATCT CATTAATACA
  781 CGTAAAGTGC CCATTAACTG GCAAACTGGA GAACAAGCTA GTCTAGAGGA AGGGGAGAAT
  841 TGTATTGACT TCTAATCAAA TTTCCCAGGG TTCATTTTGG TTGAAGCAAG GATTCTATTT
901 GAAGGATTCT TTTCTATGGG ATGGGACATT TAGGAGTGTT GGTTATTCTG GCC
//
Primer: Reverse2
Sequence file: RDB08667_A4Iib.seq check
>08667_08667_A4Ii_Reverse2_G09_21.ab1
    1 GGAGGGGCAC ATCAGGCTGC AGCGATTCGC CAGCTCGAAT TAACCCTCAC TAAAGGGAAC
   61 AAAAGCTGGT ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT
  121 CAAAAATAAA TATTTGAAAG ATGTGTTGGC GCAAACGAGT GTTACTACAA ATTTTAACGA
  181 AATAACGAAT ACGGATGAAA CAATAACAAC AAAGAGGGTA CCGAACCCAA ACTTCCTAGG
  241 AAATTGTAAC ATTAAGGATT TTAAACCTCC ATACATTTAT TCTCATGTGA ACAAAGTACC
  301 ACAAAATGTC GCTGGGGATA AAACGGCTGT TAAGCTGGAC ACTGAAGTAA AGAACACAAA
  361 TTCTAATCCA GTGGTGGCGA CGGATCCGGT CGCCGCTAAA CCGGACAACC TGGCTAACTT
  421 CAGCAACGAA GTAGCTATGA ATAATTGAAA GAAGCAAAAC ATAATGTAGG GAACACTCCA
  481 ATAAGAAATA AACCATTGTT GAAGTAGTAA TATAAACTAT CGTTCGTTAT TTTATAAAAT
  541 ATGTATTGTT ACATATATAT ACATATATAG GGAAGTGCAC AATTGGTTTG AGTGGAAGGA
  601 ATAATCCAAA ATCATAGCAG TTTGGTCTTT ATGATCTCTT GTCTGATTCT GAATGTGTAA
  661 TATGCCATAA CCAACATGAT GAAAATCAAT GGAAGTAACC AAATCATTAG TTTTCTGGCA
  721 ATTTCGTCGA CCTGAGGACC ATCTTGTCCA TGACGTTTGG TAGCTTCCCT ATACTTTTCT
  781 TGTTCAACCA GGACTTTCTC CAATTTCTGA TTGATTGATA AGAAGTCTTT GAAATTTTCT
  841 TTATCCTCAG CGATTGCATC ATCGGTGGAC GTTCCCTTTT CATTATTAGC TGGTGGCTTA
  901 GAAGAGAGCT GTTTAGTTAT CGTAGTCATG AATGATAATA GCTCTTGTTG GACCTTAATG
  961 ACATCATTTA GCTTTGTTTC TAAAGCATTG ATATCGTTCG CAGGAATTTT ACCTTCAACT
 1021 CTATCCAGTT TCTTATATAA ATTCATAGGT TTGTAATTTT GTCTTTGTCA GCGTCAAATG
 1081 CTGTTTTTTT TCCAATCATT TTTTCCTTGC GGTTTGTGGT TCAATGGAAT TGGTTGATCA
1141 GCTTTGGCCT GACATTGGCA ATCCACATTA AGGGGGAATA TCTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sato, K., Emp47p and its close homolog Emp46p have a tyrosine-containing endoplasmic reticulum exit signal and function in glycoprotein secretion in Saccharomyces cerevisiae. Mol. Biol. Cell 13, 2518-2532 (2002). PMID 12134087. [link to RRC of NBRP]