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pKSE203

Plasmid clone for production of Strep- and CFP-tagged cytosolic domain of Sed5p protein in E. coli

Catalog number RDB08713
Resource name pKSE203
Alternative name 169
Clone info. Plasmid clone for production of Strep- and CFP-tagged cytosolic domain of Sed5p protein in E. coli.
Comment MBP-CFP-UFE1 on pMAL-p2
Vector backbone pMAL-p2 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae UFE1
Depositor|Developer Nakano, Akihiko |
Other clones in our bank
External Database
S. cerevisiae UFE1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature Struct. Mol. Biol. 12, 167-174, 2005).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature Struct. Mol. Biol. 12, 167-174, 2005)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB08713 pKSE203 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Sequence RDB08713z.seq
          Remarks, protocol and/or map (pdf) RDB08713.pdf

          Original reference

          original Sato, K., Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nature Struct. Mol. Biol. 12, 167-174 (2005). PMID 15665868.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Sato, K., Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nature Struct. Mol. Biol. 12, 167-174 (2005). PMID 15665868.

          2022.07.13

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