Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein in S. cerevisiae
Alternative name | 87 |
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Clone info. | Plasmid clone to express chimeric MF(alpha)1-SEC71-WBP1 protein tagged with 3xHA under the control of SEC63 promoter in S. cerevisiae. SSTN substitution. |
Comment | MF(alpha)1-3HA-Wbp1(lum)-Sec71(tmd)-Wbp1(cyt,SSTN) (SEC63 promoter) on pRS316 (pCEN, URA3) |
Vector backbone | pRS316 (Plasmid) |
Selectable markers | Amp |
Gene/insert name | S. cerevisiae WBP1 S. cerevisiae Sec71 S. cerevisiae MF(ALPHA)1 |
Depositor|Developer | Nakano, Akihiko | |
Sequence | RDB08631z.seq | Remarks, protocol and/or map (pdf) | RDB08631.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() MTA, for use for for-profit purpose [Word ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003). |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 提供同意書 (MTA, 営利目的用)[Word ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Cell. 14, 3605-3616, 2003)。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB08631 | 316-MW71WS | Under QC test. Please contact us. | DNA solution |
Materials & Methods section:
The 316-MW71WS was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08631). |
Reference section:
Sato, K., Sato, M., Nakano, A. , Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP] |
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