Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYTY103

Plasmid clone to express ERG6 fused with mRFP in S cerevisiae

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 143, 803-811, 2008).
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem. 143, 803-811, 2008)。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB08716_A8Lcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH1pro_F () Sequence file: RDB08716_A8Lca.seq
>08716_08716_A8Lc_3_ADH1pro_F_A07_01_ABI24.ab1     1 GTCCAGTTTA TTTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT CTTCCTTGTT    61 TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTATC TCATATACAG   121 AATTCTTCGG GTGTTTTCTC CTATCCTCTG CTGCTCTCTT TTTCTTTCCA TTCGTCTTTG   181 TAATTTATAA GCTGTTAATT TTCATTCAAG TTTCCCTTAT CTGTTTTACT TTCGATTTAA   241 GTTTTACATA ATTTAAAAAA ACAAGAATAA AATAATAATA TAGTAGGCAG CATAAGATGA   301 GTGAAACAGA ATTGAGAAAA AGACAGGCCC AATTCACTAG GGAGTTACAT GGTGATGATA   361 TTGGTAAAAA GACAGGTTTG AGTGCATTGA TGTCGAAGAA CAACTCTGCC CAAAAGGAAG   421 CCGTTCAGAA GTACTTGAGA AATTGGGATG GTAGAACCGA TAAAGATGCC GAAGAACGTC   481 GTCTTGAGGA TTATAATGAA GCCACACATT CCTACTATAA CGTCGTTACA GATTTCTATG   541 AATATGGTTG GGGTTCCTCT TTCCATTTCA GCAGATTTTA TAAAGGTGAG AGTTTCGCTG   601 CCTCGATAGC AAGACATGAA CATTATTTAG CTTACAAGGC TGGTATTCAA AGAGGCGATT   661 TAGTTCTCGA CGTTGGTTGT GGTGTTGGGG GCCCAGCAAG AGAGATTGCA AGATTTACCG   721 GTTGTAACGT CATCGGTCTA AACAATAACG ATTACCAAAT TGCCAAGGCA AAATATTACG   781 CTAAAAAATA CAATTTGAGT GACCAAATGG ACTTTGTAAA GGGTGATTTC ATGAAAATGG   841 ATTTCGAAGA AAACACTTTC GACAAAGTTT ATGCAATTGA GGCCACATGT CACGCTCCAA   901 AATTAGAAAG GTGTATACAG CGAAATCTAC AAGGTTTTGA AACCGGGTGG TACCTTTGCT   961 GTTTACGAAA TGGGTAATGA CTGATAAATA TGACGAAAAA CATCCTGAAC ATAGAAAGAT  1021 CGCTTATGAA ATTGAACTAG GTGAATGGTA ATCCCAAGAT GTTCCATGTC GGACGTTGCT 1081 TAGGAAGCAA TTGAAGAACC CTGGTGGGTT C //
Primer: CMK1ter_R () Sequence file: RDB08716_A8Lcb.seq
>08716_08716_A8Lc_3_CMK1ter_R_B07_04_ABI24.ab1     1 TGGTTCCCGG GTTGTGTCAG GGCATGCACT GTGTTAACTA ATTGTGCAAA AGCTTAGATC    61 CTTAGGCGCC GGTGGAGTGG CGGCCCTCGG CGCGCTCGTA CTGTTCCACG ATGGTGTAGT   121 CCTCGTTGTG GGAGGTGATG TCCAGCTTGA TGTCGGTCTT GTAGGCGCCG GGCAGCTGCA   181 CGGGCTTCTT GGCCATGTAG GTGGTCTTGA CCTCGGCGTC GTAGTGGCCG CCGTCCTTCA   241 GCTTCAGCCT CATCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG   301 TGGAGGCCTC CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG   361 TGCCGCGCAG CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA   421 CGGTCACCAC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA   481 AGGACAGCTT CAAGTAGTCG GGGATGTCGG CGGGGTGCTT CACGTAGGCC TTGGAGCCGT   541 ACTGGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG GGGGCCGCCC TTGGTCACCT   601 TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC GCCCTCGCCC TCGATCTCGA   661 ACTCGTGGCC GTTCACGGAG CCCTCCATGC GCACCTTGAA GCGCATGAAC TCCTTGATGA   721 CGTCCTCGGA GGAGGCCATA GATCTTTGAG TTGCTTCTTG GGAAGTTTGG GAGGGGGTTT   781 CGGCGTTTTC TGGCTTCCTA GCGACGAAAA GCATCATTGG AGTGAATAAC TTGGACTTAC   841 CACCGGCAAC TAAACCAACC GCAGCATTTT CTAGAGCAGC AGTAACTTCC TTGGAACCTT   901 CTGGGGGCTA GACCTAATTT TTTCCATTAC AGTAACCATT GCTGTAGTAA ATTGTCTACC   961 CAAGTAAGAA GTTTCTGAAA AATGTGGGCC CAAATTTAGC TAAAGTTTTG AACGTACTTT  1021 CCACTCACAG TAATTGGGGT TAATACCAAC GGATTCAATC AATCATGGTT CGTCAAGGTC 1081 CACGCTTAAC GGAAGGAACT TCGAAACCCA CCAGGGTC //
Primer: URA3pro_F () Sequence file: RDB08716_A8Lcc.seq
>08716_08716_A8Lc_3_URA3pro_F_C07_07_ABI24.ab1     1 TAAAAGGCTA GTGTTGAGAC ATGAAATTGC CCAGTATTCT TAACCCAACT GCACAGAACA    61 AAAACCTGCA GGAAACGAAG ATAAATCATG TCGAAAGCTA CATATAAGGA ACGTGCTGCT   121 ACTCATCCTA GTCCTGTTGC TGCCAAGCTA TTTAATATCA TGCACGAAAA GCAAACAAAC   181 TTGTGTGCTT CATTGGATGT TCGTACCACC AAGGAATTAC TGGAGTTAGT TGAAGCATTA   241 GGTCCCAAAA TTTGTTTACT AAAAACACAT GTGGATATCT TGACTGATTT TTCCATGGAG   301 GGCACAGTTA AGCCGCTAAA GGCATTATCC GCCAAGTACA ATTTTTTACT CTTCGAAGAC   361 AGAAAATTTG CTGACATTGG TAATACAGTC AAATTGCAGT ACTCTGCGGG TGTATACAGA   421 ATAGCAGAAT GGGCAGACAT TACGAATGCA CACGGTGTGG TGGGCCCAGG TATTGTTAGC   481 GGTTTGAAGC AGGCGGCAGA AGAAGTAACA AAGGAACCTA GAGGCCTTTT GATGTTAGCA   541 GAATTGTCAT GCAAGGGCTC CCTATCTACT GGAGAATATA CTAAGGGTAC TGTTGACATT   601 GCGAAGAGCG ACAAAGATTT TGTTATCGGC TTTATTGCTC AAAGAGACAT GGGTGGAAGA   661 GATGAAGGTT ACGATTGGTT GATTATGACA CCCGGTGTGG GTTTAGATGA CAAGGGAGAC   721 GCATTGGGTC AACAGTATAG AACCGTGGAT GATGTGGTCT CTACAGGATC TGACATTATT   781 ATTGTTGGAA GAGGACTATT TGCAAAGGGA AGGGATGCTA AGGTAGAGGG TGAACGTTAC   841 AGAAAAGCAG GCTGGGAAGC ATATTTGAGA AGATGCGGCC AGCAAAACTA AAAAAACTGT   901 ATTATAAGTA AATGCATGTA TACTAAACTC ACAAATTTAG AGCTTCAATT TAATTATATC   961 AGTTATTACC CTATGCGGGT GTGAAATACC GCACAGATGC GTAAAGGAGA AAAATACCGC  1021 ATCAGAAATT GTTAAGCGTT ATATTTTTGT TTAAAAACTC GCGATAATTT TTGTAATCAG 1081 CTCATTTTTT AACCGATTAG GCCGAAATTC GGCCAAATTG C //
Primer: URA3pro_R () Sequence file: RDB08716_A8Lcd.seq
>08716_08716_A8Lc_3_URA3pro_R_D07_10_ABI24.ab1     1 CGGTCTTTCT CACACTACAT ATGCGTATAT ATACCAATCT AAGTCTGTGC TCCTTCCTTC    61 GTTCTTCCTT CTGTTCGGAG ATTACCGAAT CAAAAAAATT TCAAGGAAAC CGAAATCAAA   121 AAAAAGAATA AAAAAAAAAT GATGAATTGA AAAGGTGGTA TGGTGCACTC TCAGTACAAT   181 CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC   241 CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG   301 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT   361 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGGATCGCTT   421 GCCTGTAACT TACACGCGCC TCGTATCTTT TAATGATGGA ATAATTTGGG AATTTACTCT   481 GTGTTTATTT ATTTTTATGT TTTGTATTTG GATTTTAGAA AGTAAATAAA GAAGGTAGAA   541 GAGTTACGGA ATGAAGAAAA AAAAATAAAC AAAGGTTTAA AAAATTTCAA CAAAAAGCGT   601 ACTTTACATA TATATTTATT AGACAAGAAA AGCAGATTAA ATAGATATAC ATTCGATTAA   661 CGATAAGTAA AATGTAAAAT CACAGGATTT TCGTGTGTGG TCTTCTACAC AGACAAGATG   721 AAACAATTCG GCATTAATAC CTGAGAGCAG GAAGAGCAAG ATAAAAGGTA GTATTTGTTG   781 GCGATCCCCC TAGAGTCTTT TACATCTTCG GAAAACAAAA ACTATTTTTT CTTTAATTTC   841 TTTTTTTACT TTCTATTTTT AATTTATATA TTTATATTAA AAAAATTTAA ATTATAATTT   901 ATTTTTTATA GCACGTGATG AAAAGGACCC AGGTGGCACT TTTTCGGGGG AAAATGTGCG   961 CGGAAACCCC TATTTGTTTA TTTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA  1021 CAATAACCCT GATAAATGCT TCATATATTG AAAAATGAGA GTATGAGTAT TCACATTTCC 1081 GTGTCGCGTA ATCCGTTTTT TGCGCATTTT GGCCTTTCCT GATTTTTTGA CT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.06.19

GNP_filter3_RDBDEP_html_230615.pl