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pYTY103

Plasmid clone to express ERG6 fused with mRFP in S cerevisiae

Catalog number RDB08716
Resource name pYTY103
Alternative name 172
Clone info. Plasmid clone to express ERG6 fused with mRFP under its own promoter in S cerevisiae.
Comment ERG6-mRFP on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae ERG6
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae ERG6

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 143, 803-811, 2008).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem. 143, 803-811, 2008)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08716 pYTY103 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08716z.seq
          Remarks, protocol and/or map (pdf) RDB08716.pdf

          Original reference

          original Takeda, Y., In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. J. Biochem. 143, 803-811 (2008). PMID 18343875.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08716_A8Lcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: ADH1pro_F
          Sequence file: RDB08716_A8Lca.seq check
          >08716_08716_A8Lc_3_ADH1pro_F_A07_01_ABI24.ab1
              1 GTCCAGTTTA TTTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT CTTCCTTGTT
             61 TCTTTTTCTG CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTATC TCATATACAG
            121 AATTCTTCGG GTGTTTTCTC CTATCCTCTG CTGCTCTCTT TTTCTTTCCA TTCGTCTTTG
            181 TAATTTATAA GCTGTTAATT TTCATTCAAG TTTCCCTTAT CTGTTTTACT TTCGATTTAA
            241 GTTTTACATA ATTTAAAAAA ACAAGAATAA AATAATAATA TAGTAGGCAG CATAAGATGA
            301 GTGAAACAGA ATTGAGAAAA AGACAGGCCC AATTCACTAG GGAGTTACAT GGTGATGATA
            361 TTGGTAAAAA GACAGGTTTG AGTGCATTGA TGTCGAAGAA CAACTCTGCC CAAAAGGAAG
            421 CCGTTCAGAA GTACTTGAGA AATTGGGATG GTAGAACCGA TAAAGATGCC GAAGAACGTC
            481 GTCTTGAGGA TTATAATGAA GCCACACATT CCTACTATAA CGTCGTTACA GATTTCTATG
            541 AATATGGTTG GGGTTCCTCT TTCCATTTCA GCAGATTTTA TAAAGGTGAG AGTTTCGCTG
            601 CCTCGATAGC AAGACATGAA CATTATTTAG CTTACAAGGC TGGTATTCAA AGAGGCGATT
            661 TAGTTCTCGA CGTTGGTTGT GGTGTTGGGG GCCCAGCAAG AGAGATTGCA AGATTTACCG
            721 GTTGTAACGT CATCGGTCTA AACAATAACG ATTACCAAAT TGCCAAGGCA AAATATTACG
            781 CTAAAAAATA CAATTTGAGT GACCAAATGG ACTTTGTAAA GGGTGATTTC ATGAAAATGG
            841 ATTTCGAAGA AAACACTTTC GACAAAGTTT ATGCAATTGA GGCCACATGT CACGCTCCAA
            901 AATTAGAAAG GTGTATACAG CGAAATCTAC AAGGTTTTGA AACCGGGTGG TACCTTTGCT
            961 GTTTACGAAA TGGGTAATGA CTGATAAATA TGACGAAAAA CATCCTGAAC ATAGAAAGAT
           1021 CGCTTATGAA ATTGAACTAG GTGAATGGTA ATCCCAAGAT GTTCCATGTC GGACGTTGCT
          1081 TAGGAAGCAA TTGAAGAACC CTGGTGGGTT C
          //
          Primer: CMK1ter_R
          Sequence file: RDB08716_A8Lcb.seq check
          >08716_08716_A8Lc_3_CMK1ter_R_B07_04_ABI24.ab1
              1 TGGTTCCCGG GTTGTGTCAG GGCATGCACT GTGTTAACTA ATTGTGCAAA AGCTTAGATC
             61 CTTAGGCGCC GGTGGAGTGG CGGCCCTCGG CGCGCTCGTA CTGTTCCACG ATGGTGTAGT
            121 CCTCGTTGTG GGAGGTGATG TCCAGCTTGA TGTCGGTCTT GTAGGCGCCG GGCAGCTGCA
            181 CGGGCTTCTT GGCCATGTAG GTGGTCTTGA CCTCGGCGTC GTAGTGGCCG CCGTCCTTCA
            241 GCTTCAGCCT CATCTTGATC TCGCCCTTCA GGGCGCCGTC CTCGGGGTAC ATCCGCTCGG
            301 TGGAGGCCTC CCAGCCCATG GTCTTCTTCT GCATTACGGG GCCGTCGGAG GGGAAGTTGG
            361 TGCCGCGCAG CTTCACCTTG TAGATGAACT CGCCGTCCTG CAGGGAGGAG TCCTGGGTCA
            421 CGGTCACCAC GCCGCCGTCC TCGAAGTTCA TCACGCGCTC CCACTTGAAG CCCTCGGGGA
            481 AGGACAGCTT CAAGTAGTCG GGGATGTCGG CGGGGTGCTT CACGTAGGCC TTGGAGCCGT
            541 ACTGGAACTG AGGGGACAGG ATGTCCCAGG CGAAGGGCAG GGGGCCGCCC TTGGTCACCT
            601 TCAGCTTGGC GGTCTGGGTG CCCTCGTAGG GGCGGCCCTC GCCCTCGCCC TCGATCTCGA
            661 ACTCGTGGCC GTTCACGGAG CCCTCCATGC GCACCTTGAA GCGCATGAAC TCCTTGATGA
            721 CGTCCTCGGA GGAGGCCATA GATCTTTGAG TTGCTTCTTG GGAAGTTTGG GAGGGGGTTT
            781 CGGCGTTTTC TGGCTTCCTA GCGACGAAAA GCATCATTGG AGTGAATAAC TTGGACTTAC
            841 CACCGGCAAC TAAACCAACC GCAGCATTTT CTAGAGCAGC AGTAACTTCC TTGGAACCTT
            901 CTGGGGGCTA GACCTAATTT TTTCCATTAC AGTAACCATT GCTGTAGTAA ATTGTCTACC
            961 CAAGTAAGAA GTTTCTGAAA AATGTGGGCC CAAATTTAGC TAAAGTTTTG AACGTACTTT
           1021 CCACTCACAG TAATTGGGGT TAATACCAAC GGATTCAATC AATCATGGTT CGTCAAGGTC
          1081 CACGCTTAAC GGAAGGAACT TCGAAACCCA CCAGGGTC
          //
          Primer: URA3pro_F
          Sequence file: RDB08716_A8Lcc.seq check
          >08716_08716_A8Lc_3_URA3pro_F_C07_07_ABI24.ab1
              1 TAAAAGGCTA GTGTTGAGAC ATGAAATTGC CCAGTATTCT TAACCCAACT GCACAGAACA
             61 AAAACCTGCA GGAAACGAAG ATAAATCATG TCGAAAGCTA CATATAAGGA ACGTGCTGCT
            121 ACTCATCCTA GTCCTGTTGC TGCCAAGCTA TTTAATATCA TGCACGAAAA GCAAACAAAC
            181 TTGTGTGCTT CATTGGATGT TCGTACCACC AAGGAATTAC TGGAGTTAGT TGAAGCATTA
            241 GGTCCCAAAA TTTGTTTACT AAAAACACAT GTGGATATCT TGACTGATTT TTCCATGGAG
            301 GGCACAGTTA AGCCGCTAAA GGCATTATCC GCCAAGTACA ATTTTTTACT CTTCGAAGAC
            361 AGAAAATTTG CTGACATTGG TAATACAGTC AAATTGCAGT ACTCTGCGGG TGTATACAGA
            421 ATAGCAGAAT GGGCAGACAT TACGAATGCA CACGGTGTGG TGGGCCCAGG TATTGTTAGC
            481 GGTTTGAAGC AGGCGGCAGA AGAAGTAACA AAGGAACCTA GAGGCCTTTT GATGTTAGCA
            541 GAATTGTCAT GCAAGGGCTC CCTATCTACT GGAGAATATA CTAAGGGTAC TGTTGACATT
            601 GCGAAGAGCG ACAAAGATTT TGTTATCGGC TTTATTGCTC AAAGAGACAT GGGTGGAAGA
            661 GATGAAGGTT ACGATTGGTT GATTATGACA CCCGGTGTGG GTTTAGATGA CAAGGGAGAC
            721 GCATTGGGTC AACAGTATAG AACCGTGGAT GATGTGGTCT CTACAGGATC TGACATTATT
            781 ATTGTTGGAA GAGGACTATT TGCAAAGGGA AGGGATGCTA AGGTAGAGGG TGAACGTTAC
            841 AGAAAAGCAG GCTGGGAAGC ATATTTGAGA AGATGCGGCC AGCAAAACTA AAAAAACTGT
            901 ATTATAAGTA AATGCATGTA TACTAAACTC ACAAATTTAG AGCTTCAATT TAATTATATC
            961 AGTTATTACC CTATGCGGGT GTGAAATACC GCACAGATGC GTAAAGGAGA AAAATACCGC
           1021 ATCAGAAATT GTTAAGCGTT ATATTTTTGT TTAAAAACTC GCGATAATTT TTGTAATCAG
          1081 CTCATTTTTT AACCGATTAG GCCGAAATTC GGCCAAATTG C
          //
          Primer: URA3pro_R
          Sequence file: RDB08716_A8Lcd.seq check
          >08716_08716_A8Lc_3_URA3pro_R_D07_10_ABI24.ab1
              1 CGGTCTTTCT CACACTACAT ATGCGTATAT ATACCAATCT AAGTCTGTGC TCCTTCCTTC
             61 GTTCTTCCTT CTGTTCGGAG ATTACCGAAT CAAAAAAATT TCAAGGAAAC CGAAATCAAA
            121 AAAAAGAATA AAAAAAAAAT GATGAATTGA AAAGGTGGTA TGGTGCACTC TCAGTACAAT
            181 CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC
            241 CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG
            301 CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT
            361 GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGGATCGCTT
            421 GCCTGTAACT TACACGCGCC TCGTATCTTT TAATGATGGA ATAATTTGGG AATTTACTCT
            481 GTGTTTATTT ATTTTTATGT TTTGTATTTG GATTTTAGAA AGTAAATAAA GAAGGTAGAA
            541 GAGTTACGGA ATGAAGAAAA AAAAATAAAC AAAGGTTTAA AAAATTTCAA CAAAAAGCGT
            601 ACTTTACATA TATATTTATT AGACAAGAAA AGCAGATTAA ATAGATATAC ATTCGATTAA
            661 CGATAAGTAA AATGTAAAAT CACAGGATTT TCGTGTGTGG TCTTCTACAC AGACAAGATG
            721 AAACAATTCG GCATTAATAC CTGAGAGCAG GAAGAGCAAG ATAAAAGGTA GTATTTGTTG
            781 GCGATCCCCC TAGAGTCTTT TACATCTTCG GAAAACAAAA ACTATTTTTT CTTTAATTTC
            841 TTTTTTTACT TTCTATTTTT AATTTATATA TTTATATTAA AAAAATTTAA ATTATAATTT
            901 ATTTTTTATA GCACGTGATG AAAAGGACCC AGGTGGCACT TTTTCGGGGG AAAATGTGCG
            961 CGGAAACCCC TATTTGTTTA TTTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA
           1021 CAATAACCCT GATAAATGCT TCATATATTG AAAAATGAGA GTATGAGTAT TCACATTTCC
          1081 GTGTCGCGTA ATCCGTTTTT TGCGCATTTT GGCCTTTCCT GATTTTTTGA CT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takeda, Y., In vitro formation of a novel type of membrane vesicles containing Dpm1p: putative transport vesicles for lipid droplets in budding yeast. J. Biochem. 143, 803-811 (2008). PMID 18343875.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl