Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

316-SEC7-mRFP (#RDB08663)

Plasmid clone of trans-Golgi marker SEC7-mRFP in S. cerevisiae

Alternative name 119
Clone info. Plasmid clone to express SEC7-mRFP (ADH1 promoter) in S. cerevisiae. This clone was used as a trans-Golgi marker (Fig.3 of Osberg, C., Sci. Rep. 6: 31627, 2016).
Comment SEC7-mRFP (ADH1 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SEC7
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08663z.seq
Remarks, protocol and/or map (pdf) RDB08663.pdf

Distribution information

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Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature 441,1007-1010, 2006)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08663 316-SEC7-mRFP DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 316-SEC7-mRFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08663).

Reference section:

Matsuura-Tokita, K., Takeuchi, M., Ichihara, A., Mikuriya, K., Nakano, A., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08663_A4Io.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHpro_C
Sequence file: RDB08663_A4Ioa.seq check
>08663_08663_A4Io_ADHpro_C_G05_20.ab1
    1 ATCGGAAGTC GCGGCCGCAG ATCCATGTCT GACAGAATTC AGTAGTAAAT GCTGAAAAAG
   61 GGGATGGCGA AATATCTTCA AATGTAGAGA CTGCTTCTTC AGTCAATCCG TCTGTGAAAC
  121 CACAGAATGC TATTAAAGAA GAAGCTAAAG AGACAAATGG TGAAGATCAA AAATGCAAAG
  181 GGCCAGAAAA CGCTGGCAGT ACGGCAGAAA CAAAAGAAAC AAGTAACGAC GCTACCAACG
  241 GAATGAAAAC GCCCGAAGAA ACGGAAGATA CAAATGACAA ACGCCATGAC GATGAAGGTG
  301 AAGATGGAGA TGAAGATGAA GATGAAGATG AAGATGAAGA TGAAGACAAT GGGGACGAGG
  361 ATGATGAGGA CGTGGATAGC AGTAGTTCCG AAACCAGTTC CGAAGACGGG GAAGATTCTG
  421 AATCAGTCTC AGGGGAAAGT ACTGAGAGTA GCTCTGGAGA AGATGAAGAA TCTGATGAAA
  481 GCGATGGAAA TACATCAAAC AGCTCCTCTG GTGATGAAAG CGGGTCAGAA GAAGAAGAAG
  541 AAGAAGAAGA AGAAGAAGAA GAAGAGGAAA ATGCTGGCGA ACCAGCTATC GCTCATCAAG
  601 ATAGTGTTCC CACCAACGAT TCTACTGCCC CAAGGTCTAC CCATACGAGG AACATATCAC
  661 TATCTTCAAA TGGTTCGAAC ACAAACTCAA CCATAATTTT AGTGAAAACT ACGTTGGAGA
  721 CAATTTTAAA TGATAAGGAC ATTAAAAAGA ATTCGAATGC TCAGAAGGCT ATCGAAAGGA
  781 CATTACAAAA ATTTAAGGAA TTTGATCCGC AAACCACGAA TAACCCACAT TACGTGGATT
  841 CAATACTAGT ATTCGAAGCA CTAAGGGCAA GCTGTCGTAC CAAATCCTCC AAAGTTCAAA
  901 GTTTGGCTTT AGATTGCCTG TCAAAATTGT TTTCTTTTAG ATCGCTAGAC GAGACCCTGT
  961 TTAGTGAATC CACCCGATTC TTTTAGCCTC TAATGATCAA CGACAGATGC TGCCGATGGG
 1021 AATAACGCCG CCTCCAAAAC AAAAAATATA GACGCTGCAT TGATACTATT TCAGACTGTT
1081 TTTCAAGGTG CAGGCACTTG AATGAACGTG GTGACTACTA AATCGGTTAG GAGCCATT
//
Primer: CMK1ter_R
Sequence file: RDB08663_A4Iob.seq check
>08663_08663_A4Io_CMK1ter_R_H05_23.ab1
    1 ATTTAAATTT TTCCTTTTCG GCAGCACTGT GTTAACTAAT TGTGCAAAAG CTTAGATCCT
   61 TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG CGCTCGTACT GTTCCACGAT GGTGTAGTCC
  121 TCGTTGTGGG AGGTGATGTC CAGCTTGATG TCGGTCTTGT AGGCGCCGGG CAGCTGCACG
  181 GGCTTCTTGG CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC
  241 TTCAGCCTCA TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGTG
  301 GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG
  361 CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG
  421 GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG
  481 GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC
  541 TGGAACTGAG GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC
  601 AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC
  661 TCGTGGCCGT TCACGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC CTTGATGACG
  721 TCCTCGGAGG AGGCCATAGA TCCATCAGTA GAAAGGTATA ATTCACCAAC TCTGCTTAGA
  781 AATTGTTTTA TAGCATGTCT CAACTCCGTT GGAACGCTCT TATCTAATAT TTGAATCACC
  841 AGAGCATACA TAGCAGGACA GTATAGTCTA AAGTCTTCAT CATCAAATTC GTAGTAACCT
  901 TGTAATATTT CAACTATCAC TGAACGCCAG GCATTGATAC TACGTTCCAT GGGTTCTATC
  961 ATCCAAAGAA ACATAACCCC TCTACGACCT GCATGCAAAT GGGTGATCAG ACGAGTTATT
 1021 AAATCAGCCC TTTCTTCTCA TCATCGTTTC AGATACACTG AAAACAATAA TGTCACGAGA
 1081 ACTGCAGCAG TCACTCGGTT TCCTGGATTC CACAGGCTTG GTAATTTTTT TATTTCAAAC
1141 CCTTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]
user_report Osberg, C., Microscopy-based Saccharomyces cerevisiae complementation model reveals functional conservation and redundancy of N-terminal acetyltransferases. Sci. Rep. 6: 31627 (2016). PMID 27555049. [link to RRC of NBRP]