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316-SEC7-mRFP

Plasmid clone of trans-Golgi marker SEC7-mRFP in S. cerevisiae

Catalog number RDB08663
Resource name 316-SEC7-mRFP
Alternative name 119
Clone info. Plasmid clone to express SEC7-mRFP (ADH1 promoter) in S. cerevisiae. This clone was used as a trans-Golgi marker (Fig.3 of Osberg, C., Sci. Rep. 6: 31627, 2016).
SEC7-mRFP (ADH1 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SEC7
Depositor|Developer Nakano, Akihiko |
 
Other clones in our bank S. cerevisiae SEC7 (KEGG orthology K18442) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).
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Remarks
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08663 316-SEC7-mRFP DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB08663z.seq
Electronic file Remarks, protocol and/or map (pdf) RDB08663.pdf

Original reference

original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Akihiko Nakano's S. cerevisiae clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08663_A4Io.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: ADHpro_C
Sequence file: RDB08663_A4Ioa.seq check
>08663_08663_A4Io_ADHpro_C_G05_20.ab1
    1 ATCGGAAGTC GCGGCCGCAG ATCCATGTCT GACAGAATTC AGTAGTAAAT GCTGAAAAAG
   61 GGGATGGCGA AATATCTTCA AATGTAGAGA CTGCTTCTTC AGTCAATCCG TCTGTGAAAC
  121 CACAGAATGC TATTAAAGAA GAAGCTAAAG AGACAAATGG TGAAGATCAA AAATGCAAAG
  181 GGCCAGAAAA CGCTGGCAGT ACGGCAGAAA CAAAAGAAAC AAGTAACGAC GCTACCAACG
  241 GAATGAAAAC GCCCGAAGAA ACGGAAGATA CAAATGACAA ACGCCATGAC GATGAAGGTG
  301 AAGATGGAGA TGAAGATGAA GATGAAGATG AAGATGAAGA TGAAGACAAT GGGGACGAGG
  361 ATGATGAGGA CGTGGATAGC AGTAGTTCCG AAACCAGTTC CGAAGACGGG GAAGATTCTG
  421 AATCAGTCTC AGGGGAAAGT ACTGAGAGTA GCTCTGGAGA AGATGAAGAA TCTGATGAAA
  481 GCGATGGAAA TACATCAAAC AGCTCCTCTG GTGATGAAAG CGGGTCAGAA GAAGAAGAAG
  541 AAGAAGAAGA AGAAGAAGAA GAAGAGGAAA ATGCTGGCGA ACCAGCTATC GCTCATCAAG
  601 ATAGTGTTCC CACCAACGAT TCTACTGCCC CAAGGTCTAC CCATACGAGG AACATATCAC
  661 TATCTTCAAA TGGTTCGAAC ACAAACTCAA CCATAATTTT AGTGAAAACT ACGTTGGAGA
  721 CAATTTTAAA TGATAAGGAC ATTAAAAAGA ATTCGAATGC TCAGAAGGCT ATCGAAAGGA
  781 CATTACAAAA ATTTAAGGAA TTTGATCCGC AAACCACGAA TAACCCACAT TACGTGGATT
  841 CAATACTAGT ATTCGAAGCA CTAAGGGCAA GCTGTCGTAC CAAATCCTCC AAAGTTCAAA
  901 GTTTGGCTTT AGATTGCCTG TCAAAATTGT TTTCTTTTAG ATCGCTAGAC GAGACCCTGT
  961 TTAGTGAATC CACCCGATTC TTTTAGCCTC TAATGATCAA CGACAGATGC TGCCGATGGG
 1021 AATAACGCCG CCTCCAAAAC AAAAAATATA GACGCTGCAT TGATACTATT TCAGACTGTT
1081 TTTCAAGGTG CAGGCACTTG AATGAACGTG GTGACTACTA AATCGGTTAG GAGCCATT
//
Primer: CMK1ter_R
Sequence file: RDB08663_A4Iob.seq check
>08663_08663_A4Io_CMK1ter_R_H05_23.ab1
    1 ATTTAAATTT TTCCTTTTCG GCAGCACTGT GTTAACTAAT TGTGCAAAAG CTTAGATCCT
   61 TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG CGCTCGTACT GTTCCACGAT GGTGTAGTCC
  121 TCGTTGTGGG AGGTGATGTC CAGCTTGATG TCGGTCTTGT AGGCGCCGGG CAGCTGCACG
  181 GGCTTCTTGG CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC
  241 TTCAGCCTCA TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGTG
  301 GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG
  361 CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG
  421 GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG
  481 GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC
  541 TGGAACTGAG GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC
  601 AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC
  661 TCGTGGCCGT TCACGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC CTTGATGACG
  721 TCCTCGGAGG AGGCCATAGA TCCATCAGTA GAAAGGTATA ATTCACCAAC TCTGCTTAGA
  781 AATTGTTTTA TAGCATGTCT CAACTCCGTT GGAACGCTCT TATCTAATAT TTGAATCACC
  841 AGAGCATACA TAGCAGGACA GTATAGTCTA AAGTCTTCAT CATCAAATTC GTAGTAACCT
  901 TGTAATATTT CAACTATCAC TGAACGCCAG GCATTGATAC TACGTTCCAT GGGTTCTATC
  961 ATCCAAAGAA ACATAACCCC TCTACGACCT GCATGCAAAT GGGTGATCAG ACGAGTTATT
 1021 AAATCAGCCC TTTCTTCTCA TCATCGTTTC AGATACACTG AAAACAATAA TGTCACGAGA
 1081 ACTGCAGCAG TCACTCGGTT TCCTGGATTC CACAGGCTTG GTAATTTTTT TATTTCAAAC
1141 CCTTA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523.
user report Osberg, C., Microscopy-based Saccharomyces cerevisiae complementation model reveals functional conservation and redundancy of N-terminal acetyltransferases. Sci. Rep. 6: 31627 (2016). PMID 27555049.

2020.11.04

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