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316-SEC7-mRFP

Plasmid clone of trans-Golgi marker SEC7-mRFP in S. cerevisiae

Catalog number RDB08663
Resource name 316-SEC7-mRFP
Alternative name 119
Clone info. Plasmid clone to express SEC7-mRFP (ADH1 promoter) in S. cerevisiae. This clone was used as a trans-Golgi marker (Fig.3 of Osberg, C., Sci. Rep. 6: 31627, 2016).
Comment SEC7-mRFP (ADH1 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SEC7
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae SEC7

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature 441,1007-1010, 2006)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

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          RDB08663 316-SEC7-mRFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB08663z.seq
          Remarks, protocol and/or map (pdf) RDB08663.pdf

          Original reference

          original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08663_A4Io.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: ADHpro_C
          Sequence file: RDB08663_A4Ioa.seq check
          >08663_08663_A4Io_ADHpro_C_G05_20.ab1
              1 ATCGGAAGTC GCGGCCGCAG ATCCATGTCT GACAGAATTC AGTAGTAAAT GCTGAAAAAG
             61 GGGATGGCGA AATATCTTCA AATGTAGAGA CTGCTTCTTC AGTCAATCCG TCTGTGAAAC
            121 CACAGAATGC TATTAAAGAA GAAGCTAAAG AGACAAATGG TGAAGATCAA AAATGCAAAG
            181 GGCCAGAAAA CGCTGGCAGT ACGGCAGAAA CAAAAGAAAC AAGTAACGAC GCTACCAACG
            241 GAATGAAAAC GCCCGAAGAA ACGGAAGATA CAAATGACAA ACGCCATGAC GATGAAGGTG
            301 AAGATGGAGA TGAAGATGAA GATGAAGATG AAGATGAAGA TGAAGACAAT GGGGACGAGG
            361 ATGATGAGGA CGTGGATAGC AGTAGTTCCG AAACCAGTTC CGAAGACGGG GAAGATTCTG
            421 AATCAGTCTC AGGGGAAAGT ACTGAGAGTA GCTCTGGAGA AGATGAAGAA TCTGATGAAA
            481 GCGATGGAAA TACATCAAAC AGCTCCTCTG GTGATGAAAG CGGGTCAGAA GAAGAAGAAG
            541 AAGAAGAAGA AGAAGAAGAA GAAGAGGAAA ATGCTGGCGA ACCAGCTATC GCTCATCAAG
            601 ATAGTGTTCC CACCAACGAT TCTACTGCCC CAAGGTCTAC CCATACGAGG AACATATCAC
            661 TATCTTCAAA TGGTTCGAAC ACAAACTCAA CCATAATTTT AGTGAAAACT ACGTTGGAGA
            721 CAATTTTAAA TGATAAGGAC ATTAAAAAGA ATTCGAATGC TCAGAAGGCT ATCGAAAGGA
            781 CATTACAAAA ATTTAAGGAA TTTGATCCGC AAACCACGAA TAACCCACAT TACGTGGATT
            841 CAATACTAGT ATTCGAAGCA CTAAGGGCAA GCTGTCGTAC CAAATCCTCC AAAGTTCAAA
            901 GTTTGGCTTT AGATTGCCTG TCAAAATTGT TTTCTTTTAG ATCGCTAGAC GAGACCCTGT
            961 TTAGTGAATC CACCCGATTC TTTTAGCCTC TAATGATCAA CGACAGATGC TGCCGATGGG
           1021 AATAACGCCG CCTCCAAAAC AAAAAATATA GACGCTGCAT TGATACTATT TCAGACTGTT
          1081 TTTCAAGGTG CAGGCACTTG AATGAACGTG GTGACTACTA AATCGGTTAG GAGCCATT
          //
          Primer: CMK1ter_R
          Sequence file: RDB08663_A4Iob.seq check
          >08663_08663_A4Io_CMK1ter_R_H05_23.ab1
              1 ATTTAAATTT TTCCTTTTCG GCAGCACTGT GTTAACTAAT TGTGCAAAAG CTTAGATCCT
             61 TAGGCGCCGG TGGAGTGGCG GCCCTCGGCG CGCTCGTACT GTTCCACGAT GGTGTAGTCC
            121 TCGTTGTGGG AGGTGATGTC CAGCTTGATG TCGGTCTTGT AGGCGCCGGG CAGCTGCACG
            181 GGCTTCTTGG CCATGTAGGT GGTCTTGACC TCGGCGTCGT AGTGGCCGCC GTCCTTCAGC
            241 TTCAGCCTCA TCTTGATCTC GCCCTTCAGG GCGCCGTCCT CGGGGTACAT CCGCTCGGTG
            301 GAGGCCTCCC AGCCCATGGT CTTCTTCTGC ATTACGGGGC CGTCGGAGGG GAAGTTGGTG
            361 CCGCGCAGCT TCACCTTGTA GATGAACTCG CCGTCCTGCA GGGAGGAGTC CTGGGTCACG
            421 GTCACCACGC CGCCGTCCTC GAAGTTCATC ACGCGCTCCC ACTTGAAGCC CTCGGGGAAG
            481 GACAGCTTCA AGTAGTCGGG GATGTCGGCG GGGTGCTTCA CGTAGGCCTT GGAGCCGTAC
            541 TGGAACTGAG GGGACAGGAT GTCCCAGGCG AAGGGCAGGG GGCCGCCCTT GGTCACCTTC
            601 AGCTTGGCGG TCTGGGTGCC CTCGTAGGGG CGGCCCTCGC CCTCGCCCTC GATCTCGAAC
            661 TCGTGGCCGT TCACGGAGCC CTCCATGCGC ACCTTGAAGC GCATGAACTC CTTGATGACG
            721 TCCTCGGAGG AGGCCATAGA TCCATCAGTA GAAAGGTATA ATTCACCAAC TCTGCTTAGA
            781 AATTGTTTTA TAGCATGTCT CAACTCCGTT GGAACGCTCT TATCTAATAT TTGAATCACC
            841 AGAGCATACA TAGCAGGACA GTATAGTCTA AAGTCTTCAT CATCAAATTC GTAGTAACCT
            901 TGTAATATTT CAACTATCAC TGAACGCCAG GCATTGATAC TACGTTCCAT GGGTTCTATC
            961 ATCCAAAGAA ACATAACCCC TCTACGACCT GCATGCAAAT GGGTGATCAG ACGAGTTATT
           1021 AAATCAGCCC TTTCTTCTCA TCATCGTTTC AGATACACTG AAAACAATAA TGTCACGAGA
           1081 ACTGCAGCAG TCACTCGGTT TCCTGGATTC CACAGGCTTG GTAATTTTTT TATTTCAAAC
          1141 CCTTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523.
          user_report Osberg, C., Microscopy-based Saccharomyces cerevisiae complementation model reveals functional conservation and redundancy of N-terminal acetyltransferases. Sci. Rep. 6: 31627 (2016). PMID 27555049.

          2022.05.17

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