Resource data sheet
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pET21a-SED5 (#RDB08692)

Plasmid clone for production of 6xHis-tagged cytosolic domain of Sed5p in E. coli

Alternative name 148
Clone info. Plasmid clone for production of 6xHis-tagged cytosolic domain of Sed5p (residues 1-316) protein in E. coli.
Comment SED5 (1-316)-Hisx6 on pET21a
Vector backbone pET21a (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SED5 (1-316)
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08692z.seq
Remarks, protocol and/or map (pdf) RDB08692.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature Struct. Mol. Biol. 12, 167-174, 2005).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature Struct. Mol. Biol. 12, 167-174, 2005)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08692 pET21a-SED5 Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pET21a-SED5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08692).

Reference section:

Sato, K., Nakano, A., Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nature Struct. Mol. Biol. 12, 167-174 (2005). PMID 15665868. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Sato, K., Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nature Struct. Mol. Biol. 12, 167-174 (2005). PMID 15665868. [link to RRC of NBRP]