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pYO326-chimeraN (#RDB08788)

Plasmid clone for overproduction of chimera N form of ERG11 in S. cerevisiae

Alternative name 245
Clone info. Plasmid clone for overproduction of chimera N form of ERG11 in S. cerevisiae. The chimera N contains a potential N-glycosylation site at N-terminus and 3xHA-tag at C-terminus, respectively.
Comment ERG11(283-312, with a potential N-Glycosylation site)-ERG11-3HA on pYO326 (2micron, URA3)
Vector backbone pYO326 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae ERG11
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08788z.seq
Remarks, protocol and/or map (pdf) RDB08788.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biochem. 127, 747-754, 2000).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem. 127, 747-754, 2000)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB08788 pYO326-chimeraN Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pYO326-chimeraN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08788).

Reference section:

Homma, K., Yoshida, Y., Nakano, A., Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. J. Biochem. 127, 747-754 (2000). PMID 10788782. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Homma, K., Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-Golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism. J. Biochem. 127, 747-754 (2000). PMID 10788782. [link to RRC of NBRP]