Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

316-mRFP-SEC12 (#RDB08650)

Plasmid clone to express SEC12 protein tagged with mRFP under the control of TDH3 promoter in S. cerevisiae

Alternative name 106
Clone info. Plasmid clone to express SEC12 protein tagged with mRFP under the control of TDH3 promoter in S. cerevisiae.
Comment mRFP-SEC12 (TDH3 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SEC12
Depositor|Developer Nakano, Akihiko |
 
Sequence RDB08650z.seq
Remarks, protocol and/or map (pdf) RDB08650.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003).
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Cell. 14, 3605-3616, 2003)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08650 316-mRFP-SEC12 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The 316-mRFP-SEC12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08650).

Reference section:

Sato, K., Sato, M., Nakano, A. , Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08650_A4Io.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: TDH3pro_F
Sequence file: RDB08650_A4Ioa.seq check
>08650_08650_A4Io_TDH3pro_F_C04_07.ab1
    1 ATAAGGACGG TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC
   61 TACTTTTATA GTTAGTCTTT TTTTTAGTTT TAAAACACCA AGAACTTAGT TTCGAATAAA
  121 CACACATAAA CAGAATTCAT GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC
  181 AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG
  241 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG
  301 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCCAGTACG GCTCCAAGGC CTACGTGAAG
  361 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG
  421 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG
  481 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC
  541 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG AGCGGATGTA CCCCGAGGAC
  601 GGCGCCCTGA AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC
  661 GCCGAGGTCA AGACCACCTA CATGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAG
  721 ACCGACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC
  781 GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCAGATCTA TGAAGTTCGT GACAGCTAGT
  841 TATAACGTCG GGTATCCTGC GTACGGTGCA AAATTTTTGA ATAACGACAC ATTACTTGTG
  901 GCAGGCGGTG GAGGAGAAGG AAACAATGGC ATACCAAACA AGCTGACGGT CTTGCGCGTG
  961 GATCCTACCA AAGATACTGA GAAAGGAACA GTTTCATATA TTTGAGCGAG TTTGCATTGG
 1021 AAGACAACGA CGACTCTCCT ACTGCAATTG ACGCTTTCCA AGGGGTATCA TTTTTGTTTG
 1081 CCTGCATGAA AATAGGCACT TAGGATACTC TAGCTAAAGG TAATAGGCCA CTTGGGAGAA
1141 ACAAAAC
//
Primer: CMK1ter_R
Sequence file: RDB08650_A4Iob.seq check
>08650_08650_A4Io_CMK1ter_R_D04_10.ab1
    1 CTTTATTAAA TTTTTCCTTT TCGGCAGCAC TGTGTTAACT AATTGTGCAA AAGCTTAGAT
   61 CTAGCATCAT CTATTTCTCG AAAAGTTGCG TTGGTGATCT CAAACTCTGC CCCTCCAGTA
  121 TTAATAATGT TACTTTCAGT ACATAAGACT TCAGTTCCAT TCACAGAACT CGTCTCATGC
  181 ACGCCATGCA CTTTTATGGA ATCTACGCTA GGTACAGATG TTTTTGTACC GTGGTTGCCA
  241 AACAAAGTTG TTTGATGTAA GTCTTCATCA ACTACGTAGG GCGAAGAGAT GGTGTCTCTT
  301 TTCGTTAGAA AATTGTCCTT CGCGTAATTG AAAAGCATGG AATGCAAATT GTGCTTATAG
  361 GAGAACTGTA AAATGTAAGA AAGCAGCACA ATAAGGATGA AGTTGGTGAA AAATTTAGAG
  421 ATTTTTTGTT TCATTGAGGT GTAGTTGGCG TAGTTAAGCG GTAATTTTAT TATGTGGATA
  481 GTGTTGGCTG CCGAAACACT CGCCACATAT GTAGAGTCCG GAGAGATAGT GACCTCTGTA
  541 ATGGCAAAAC TATGAGCTTG TTTGAATATT TTAGACATTG ACAGGTCTTT TAGTTTCACA
  601 AGAGCTATGG AATTGTCATT ACTTGCCAGT ACCGCCAATT CACCCTTCAT GTCGACATCC
  661 ATAGAAGTAA TCCCTTTGAA TCTGTTTGTC ACTTGTTTGG ATCTTAATAC GGAAGTGTTT
  721 CCTGATTTGA TGCTTATTTT GGTCAGCACA ATACCTTTCC CTTTTTTTAA AGAGGCTGCT
  781 ATCAATACTG TGTCATCGGC TATGAAGTTT ATTTTAGATA AACTCTAATT CTTATCAAAA
  841 TCTGTTTTCC TAGCAATGCA ACTTCCAGTC ACTGTTGAAA TCACTTCCAA GCTAGAACCG
  901 GGTGATATAA GCAACAACCT TACCATCAGT GGAAAAGTGT AAATCCTTTA CTTCACCCCT
  961 AGTCTCGATC TCAAACTTCT CTGTCAAGTC GCTCGGGTCA ATGATTCTCA TTATAGCAGG
 1021 TACTTTAGAT GATGCGATAG CTGCAACGGG TACCTCTCGG TGGAAATATA ACCAGCTCGT
 1081 GTAGTTCATC CGGCATTTTG TAGAATGCGT TCAAGGTCTA ACCTAGTTGA AGAACTCATT
1141 GAATCAATTC ACTATCAATA AGTTCCACAA GAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP]