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Siro Simizu's Resource

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Catalog #Name of cloneRunning titlePublication
RDB13302pCI-ECM1Expression vector of human ECM1 cDNAUematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385. (original).
RDB13303pCI-ECM1-Q276XExpression vector of human ECM1 (Q276X) cDNAUematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385. (original).
RDB13304pCI-ECM1-W359XExpression vector of human ECM1 (W359X) cDNAUematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385. (original).
RDB13305pCI-ECM1-N354QExpression vector of human ECM1 (N354Q) cDNAUematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385. (original).
RDB13306pCI-ECM1-N444QExpression vector of human ECM1 (N444Q) cDNAUematsu, S., N-Glycosylation of extracellular matrix protein 1 (ECM1) regulates its secretion, which is unrelated to lipoid proteinosis. FEBS Open Bio. 4: 879-885 (2014). PMID 25379385. (original).
RDB13307pCI-HYAL1Expression vector of human HYAL1 cDNAGoto, Y., N-glycosylation is required for secretion and enzymatic activity of human hyaluronidase1. FEBS Open Bio. 554-559 (2014). PMID 25009769. (original).
RDB13308pCI-HYAL1-N99QExpression vector of human HYAL1 (N99Q) cDNAGoto, Y., N-glycosylation is required for secretion and enzymatic activity of human hyaluronidase1. FEBS Open Bio. 554-559 (2014). PMID 25009769. (original).
RDB13309pCI-HYAL1-N216QExpression vector of human HYAL1 (N216Q) cDNAGoto, Y., N-glycosylation is required for secretion and enzymatic activity of human hyaluronidase1. FEBS Open Bio. 554-559 (2014). PMID 25009769. (original).
RDB13310pCI-HYAL1-N350QExpression vector of human HYAL1 (N350Q) cDNAGoto, Y., N-glycosylation is required for secretion and enzymatic activity of human hyaluronidase1. FEBS Open Bio. 554-559 (2014). PMID 25009769. (original).
RDB14252pcDNA3.1/Myc-His(+)-Heparanase(HP)Expression vector of Myc-His tagged human Heparanase (HP) wild type.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14253pcDNA3.1/Myc-His(+)-HP/N162QExpression vector of Myc-His tagged human Heparanase (HP) mutant N162Q.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14254pcDNA3.1/Myc-His(+)-HP/N178QExpression vector of Myc-His tagged human Heparanase (HP) mutant N178Q.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14255pcDNA3.1/Myc-His(+)-HP/N200QExpression vector of Myc-His tagged human Heparanase (HP) mutant N200Q.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14256pcDNA3.1/Myc-His(+)-HP/N217QExpression vector of Myc-His tagged human Heparanase (HP) mutant N217Q.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14257pcDNA3.1/Myc-His(+)-HP/N238QExpression vector of Myc-His tagged human Heparanase (HP) mutant N238Q.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14258pcDNA3.1/Myc-His(+)-HP/N459QExpression vector of Myc-His tagged human Heparanase (HP) mutant N459Q.Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. (original).
RDB14259pcDNA3.1/Myc-His(+)-HP/C127SExpression vector of Myc-His tagged human Heparanase (HP) mutant C127S.Simizu, S., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. (original).
RDB14260pcDNA3.1/Myc-His(+)-HP/C179SExpression vector of Myc-His tagged human Heparanase (HP) mutant C179S.Simizu, S., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. (original).
RDB14261pcDNA3.1/Myc-His(+)-HP/C211SExpression vector of Myc-His tagged human Heparanase (HP) mutant C211S.Simizu, S., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. (original).
RDB14262pcDNA3.1/Myc-His(+)-HP/C437SExpression vector of Myc-His tagged human Heparanase (HP) mutant C437S.Simizu, S., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. (original).
RDB14263pcDNA3.1/Myc-His(+)-HP/C542SExpression vector of Myc-His tagged human Heparanase (HP) mutant C542S.Simizu, S., Involvement of disulfide bond formation in the activation of heparanase. Cancer. Res, 67 (16): 7841-7849 (2007). PMID 17699790. (original).
RDB14554pCI-neo/ARL6ip1(1-29)-Gluc-ARL6ip1(30-203) [V29]Expression vector of human ARL6ip1 plus Gluc inserted after Val at 29aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14555pCI-neo/ARL6ip1(1-37)-Gluc-ARL6ip1(38-203) [L37]Expression vector of human ARL6ip1 plus Gluc inserted after Leu at 37aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14556pCI-neo/ARL6ip1(1-64)-Gluc-ARL6ip1(65-203) [P64]Expression vector of human ARL6ip1 plus Gluc inserted after Pro at 64aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14557pCI-neo/ARL6ip1(1-64)-Gluc [P64*]Expression vector of N-terminal 64aa of human ARL6ip1 plus Gluc.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14558pCI-neo/ARL6ip1(1-90)-Gluc-ARL6ip1(91-203) [R90]Expression vector of human ARL6ip1 plus Gluc inserted after Arg at 90aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14559pCI-neo/ARL6ip1(1-99)-Gluc-ARL6ip1(100-203) [T99]Expression vector of human ARL6ip1 plus Gluc inserted after Thr at 99aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14560pCI-neo/ARL6ip1(1-119)-Gluc-ARL6ip1(120-203) [A119]Expression vector of human ARL6ip1 plus Gluc inserted after Ala at 119aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14561pCI-neo/ARL6ip1(1-189)-Gluc-ARL6ip1(190-203) [R189]Expression vector of human ARL6ip1 plus Gluc inserted after Arg at 189aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14562pCI-neo/ARL6ip1(1-199)-Gluc-ARL6ip1(200-203) [E199]Expression vector of human ARL6ip1 plus Gluc inserted after Glu at 199aa.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14563pCI-neo/GFP-ARL6ip1(WT)Expression vector of N-terminal GFP plus human ARL6ip1, wild type.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14564pCI-neo/GFP-ARL6ip1(delta1-41)Expression vector of N-terminal GFP plus human ARL6ip1, deletion mutant, delta 1-41 a.a.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14565pCI-neo/GFP-ARL6ip1(delta1-86)Expression vector of N-terminal GFP plus human ARL6ip1, deletion mutant, delta 1-86 a.a.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14566pCI-neo/GFP-ARL6ip1(delta1-133)Expression vector of N-terminal GFP plus human ARL6ip1, deletion mutant, delta 1-133 a.aKuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14567pCI-neo/GFP-ARL6ip1(delta42-86)Expression vector of N-terminal GFP plus human ARL6ip1, deletion mutant, delta 42-86 a.a.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB14568pCI-neo/GFP-ARL6ip1(42-133+KKNE)Expression vector of N-terminal GFP plus 42-133aa of human ARL6ip1.Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. (original).
RDB18689CSⅡ-CMV-MCS-IRES2-Bsd-CEACAM1 wtExpression vector of human CEACAM1.Hayashi, S., Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. (original).
RDB18690CSⅡ-CMV-MCS-IRES2-Bsd-CEACAM1 rescueExpression vector of Cas9-resistant human CEACAM1.Hayashi, S., Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. (original).
RDB18691pCI-neo MFAP4-MH wtExpression vector of human MFAP4, wild-type.Osada, Y., The Fibrinogen C-terminal Domain Is Seldom C-mannosylated but Its C-mannosylation Is Important for the Secretion of Microfibril-Associated Glycoprotein 4. Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). PMID 32442478. (original).
RDB18692pCI-neo MFAP4-HA WFExpression vector of human MFAP4, mutant W235F.Osada, Y., The Fibrinogen C-terminal Domain Is Seldom C-mannosylated but Its C-mannosylation Is Important for the Secretion of Microfibril-Associated Glycoprotein 4. Biochim. Biophys. Acta. Gen. Subj. 1864 (9): 129637 (2020). PMID 32442478. (original).
RDB19523pCI-neo-DPY19L3(wt)-Gluc(C-term)Expression vector of human DPY19L3 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19524pCI-neo-DPY19L3(isoform2)-GlucExpression vector of huamn DPY19L3 isoform2 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19525pCI-neo-DPY19L3(wt)-Gluc(N-term)Expression vector of human DPY19L3 tagged with Gluc at N-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19526pCI-neo-DPY19L3(E80)-GlucExpression vector of human DPY19L3 which C-terminus trancated at E80 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19527pCI-neo-DPY19L3(K151)-GlucExpression vector of human DPY19L3 which C-terminus trancated at K151 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19528pCI-neo-DPY19L3(G183)-GlucExpression vector of human DPY19L3 which C-terminus trancated at G183 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19529pCI-neo-DPY19L3(V205)-GlucExpression vector of human DPY19L3 which C-terminus trancated at V205 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19530pCI-neo-DPY19L3(T208)-GlucExpression vector of human DPY19L3 which C-terminus trancated at T208 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19531pCI-neo-DPY19L3(P237)-GlucExpression vector of human DPY19L3 which C-terminus trancated at P237 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19532pCI-neo-DPY19L3(Q262)-GlucExpression vector of human DPY19L3 which C-terminus trancated at Q262 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19533pCI-neo-DPY19L3(K285)-GlucExpression vector of human DPY19L3 which C-terminus trancated at K285 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19534pCI-neo-DPY19L3(N307)-GlucExpression vector of human DPY19L3 which C-terminus trancated at N307 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19535pCI-neo-DPY19L3(N339)-GlucExpression vector of human DPY19L3 which C-terminus trancated at N339 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19536pCI-neo-DPY19L3(A400)-GlucExpression vector of human DPY19L3 which C-terminus trancated at A400 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19537pCI-neo-DPY19L3(T456)-GlucExpression vector of human DPY19L3 which C-terminus trancated at T456 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19538pCI-neo-DPY19L3(L486)-GlucExpression vector of human DPY19L3 which C-terminus trancated at L486 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19539pCI-neo-DPY19L3(Y515)-GlucExpression vector of human DPY19L3 which C-terminus trancated at Y515 tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19544pCI-neo-DPY19L3(2NQ)-GlucExpression vector of human DPY19L3 N118Q/N704Q (2NQ) mutant tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19545pCI-neo-DPY19L3(2NQ+141NV-in)-GlucExpression vector of human DPY19L3 N118Q/N704Q (2NQ) mutant with a NV residue-insertion tagged with Gluc at C-terminus to analyze its ER membrane topology.Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. (original).
RDB19546pCI-neo-FGL1-MHExpression vector of human FGL1 tagged with c-Myc and His6 at C-terminus.Mori, K., Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1. Biochem. Biophys. Res. Commun. 560: 93-98 (2021). PMID 33984770. (original).
RDB19547pCI-neo-FGL1(K65R)-MHExpression vector of human FGL1 K65R mutant tagged with c-Myc and His6 at C-terminus.Mori, K., Involvement of LH3 and GLT25D1 for glucosyl-galactosyl-hydroxylation on non-collagen-like domain of FGL1. Biochem. Biophys. Res. Commun. 560: 93-98 (2021). PMID 33984770. (original).

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2022.05.18

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