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CSII-CMV-MCS-IRES2-Bsd-CEACAM1 rescue (#RDB18690)

Expression vector of Cas9-resistant human CEACAM1.

Clone info. Expression vector of Cas9-resistant human carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) tagged with Myc at C-terminus. For example of use, please refer theFig.4 of Hayashi, S. et al., Biochem. Biophys. Rep., 21: 100734, 2020.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone CSII-CMV-MCS-IRES2-Bsd (RDB04385) (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Ampicillin (E. coli)
Growth conditions LB+Ampicillin, 37oC
Growth remarks Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name human CEACAM1 cDNA
Depositor|Developer Simizu, Siro |
 
Sequence (full) RDB18690hts01.seq checkAssembled from experimentally sequenced data.
Publication Hayashi, S., Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. [link to RRC of NBRP]
Test sheet RDB18690C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Hayashi, S. et al., Biochem. Biophys. Rep., 21: 100734, 2020).
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks Remember that you will be working with samples containing infectious virus.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hayashi, S. et al., Biochem. Biophys. Rep., 21: 100734, 2020)。
付加的使用条件：
レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18690 CSII-CMV-MCS-IRES2-Bsd-CEACAM1 rescue DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]

How to cite this biological resource

Materials & Methods section:

The CSII-CMV-MCS-IRES2-Bsd-CEACAM1 rescue was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18690).

Reference section:

Hayashi, S., Osada, Y., Miura, K., Simizu, S., Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hayashi, S., Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Biochem. Biophys. Rep. 21: 100734 (2020). PMID 32025578. [link to RRC of NBRP]