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pCI-neo/GFP-ARL6ip1(delta42-86) (#RDB14567)

Expression vector of N-terminal GFP plus human ARL6ip1, deletion mutant, delta 42-86 a.a.

Clone info. Expression vector of N-terminal GFP (AcGFP) plus human ADP-ribosylation factor-like 6-interacting protein 1 (ARL6ip1) deletion mutant, delta 42-86 a.a. For the structure of the plasmid construct, see Fig. 3 of Kuroda, M. et al. FEBS Lett., 587 (22): 3656-3660, 2013.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human ARL6IP1 cDNA Aequorea coerulescens GFP cDNA
Depositor|Developer Simizu, Siro |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Masahiro, K. et al. FEBS Lett. 587, 3656-3660, 2013)
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Masahiro, K. et al. FEBS Lett. 587, 3656-3660, 2013)。
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本件リソースの使用は学術機関での学術研究に限る。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14567 pCI-neo/GFP-ARL6ip1(delta42-86) Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCI-neo/GFP-ARL6ip1(delta42-86) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14567).

Reference section:

Kuroda, M., Funasaki, S., Saitoh, T., Sasazawa, Y., Nishiyama, S., Umezawa, K., Simizu, S., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Kuroda, M., Determination of topological structure of ARL6ip1 in cells: identification of the essential binding region of ARL6ip1 for conophylline. FEBS Lett. 587 (22): 3656-3660 (2013). PMID 24076029. [link to RRC of NBRP]