Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCI-neo-DPY19L3(E80)-Gluc (#RDB19526)

Expression vector of human DPY19L3 which C-terminus trancated at E80 tagged with Gluc at C-terminus to analyze its ER membrane topology.

Clone info. Expression vector of human dpy-19 like C-mannosyltransferase 3 (DPY19L3) which C-terminus trancated at E80, tagged with Gaussia luciferase (Gluc) at C-terminus for the redox-sensitive luciferase assay. See Fig.2 of Niwa, Y. et al. FEBS J. 285 (6): 1162-1174, 2018.
Comment Expression was confirmed by the depositor with redox-sensitive luciferase assay.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name human DPY19L3 cDNA
Depositor|Developer Simizu, Siro |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Niwa, Y. et al., FEBS J. 285 (6): 1162-1174, 2018)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Niwa, Y. et al., FEBS J. 285 (6): 1162-1174, 2018)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB19526 pCI-neo-DPY19L3(E80)-Gluc Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCI-neo-DPY19L3(E80)-Gluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19526).

Reference section:

Niwa, Y., Nakano, Y., Suzuki, T., Yamagishi, M., Otani, K., Dohmae, N., Simizu, S., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Niwa, Y., Topological analysis of DPY19L3, a human C-mannosyltransferase. FEBS J. 285 (6): 1162-1174 (2018). PMID 29405629. [link to RRC of NBRP]