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pcDNA3.1/Myc-His(+)-HP/N200Q (#RDB14255)

Expression vector of Myc-His tagged human Heparanase (HP) mutant N200Q.

Clone info. Expression vector of Myc-His tagged human Heparanase (HP) mutant N200Q, see Fig. 1A of Simizu, S., J. Biol. Chem, 279 (4): 2697-703 (2004) for details.
Comment Expression was confirmed by the depositor with Western blotting and fluorescent microscopy. cDNAfragment 138nt to 1766nt of NM_006665.5 is cloned. Known differences (at least): substitutions T to C at 419nt (94aa Phe, synonym), A to G at 1,057nt (K307R), A to G at 1,166nt (343aa Glu, synonym).
Vector backbone pcDNA3.1 Myc-His(+) ()
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human HPSE cDNA
Depositor|Developer Simizu, Siro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shimizu, S. et al., J. Boil. Chem. 279:2697-2703, 2004)
提供案内 (日本国内) [open/close]

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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shimizu, S. et al., J. Boil. Chem. 279:2697-2703, 2004)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14255 pcDNA3.1/Myc-His(+)-HP/N200Q Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

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How to cite this biological resource

Materials & Methods section:

The pcDNA3.1/Myc-His(+)-HP/N200Q was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14255).

Reference section:

Simizu, S., Ishida, K., Wierzba, M.K., Osada, H., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Simizu, S., Secretion of heparanase protein is regulated by glycosylation in human tumor cell lines. J. Biol. Chem, 279 (4): 2697-703 (2004). PMID 14573609. [link to RRC of NBRP]