Expression vector of human ADAMTS4, mutant W523F, W526F, and W529F.
Clone info. | Expression vector of human A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) tagged with Myc-His6 at C-terminal, mutant W523F, W526F, and W529F. |
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Comment | Expression was confirmed by the depositor with western blotting. |
Vector backbone | pCI-neo (plasmid) |
Size of vector backbone | 5.5 kb |
Selectable markers | Ampicillin (E. coli), Neomycin (mammalian cell) |
Growth conditions | LB+Ampicillin, 37oC |
Gene/insert name | human ADAMTS4 cDNA |
Depositor|Developer | Simizu, Siro | |
Sequence (full) | RDB18694hts01.seq ![]() |
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Publication | Miura, K., Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129833 (2021). [link to RRC of NBRP] |
Test sheet | RDB18694C0p1-1.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Miura. K. et al., Biochim. Biophys. Acta-Gen. Subj., 1865 (3): 129833, 2021) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miura. K. et al., Biochim. Biophys. Acta-Gen. Subj., 1865 (3): 129833, 2021)。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB18694 | pCI-Neo ADAMTS4(3WF)-MH | DNA solution |
Materials & Methods section:
The pCI-Neo ADAMTS4(3WF)-MH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18694). |
Reference section:
Miura, K., Suzuki, T., Sun, H., Takada, H., Ishizawa, Y., Mizuta, H., Dohmae, N., Simizu, S., Requirement for C-mannosylation to be secreted and activated a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4). Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129833 (2021). PMID 33358865. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Miura, K., Requirement for C-mannosylation to be secreted and activated a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4). Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129833 (2021). PMID 33358865. [link to RRC of NBRP] |
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