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pCI-Neo ADAMTS4(3WF)-MH (#RDB18694)

Expression vector of human ADAMTS4, mutant W523F, W526F, and W529F.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Miura, K., Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129833 (2021). PMID 33358865. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of human A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4) tagged with Myc-His6 at C-terminal, mutant W523F, W526F, and W529F.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCI-neo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Ampicillin, 37oC
Gene/insert name human ADAMTS4 cDNA
Depositor|Developer Simizu, Siro |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. (Miura. K. et al., Biochim. Biophys. Acta-Gen. Subj., 1865 (3): 129833, 2021)
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Miura. K. et al., Biochim. Biophys. Acta-Gen. Subj., 1865 (3): 129833, 2021)。

Catalog # Resource name Shipping form Fee
RDB18694 pCI-Neo ADAMTS4(3WF)-MH DNA solution

How to cite this biological resource

Materials & Methods section:

The pCI-Neo ADAMTS4(3WF)-MH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18694).

Reference section:

Miura, K., Suzuki, T., Sun, H., Takada, H., Ishizawa, Y., Mizuta, H., Dohmae, N., Simizu, S., Requirement for C-mannosylation to be secreted and activated a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4). Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129833 (2021). PMID 33358865. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Miura, K., Requirement for C-mannosylation to be secreted and activated a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS4). Biochim. Biophys. Acta Gen. Subj. 1865 (3): 129833 (2021). PMID 33358865. [PubMed] [Article] [RRC of NBRP]